Table 1.
Microglial depletion and repopulation do not negatively affect animal behavior in noninjured mice, including tests of anxiety, motor, and cognitive function
| Sham + vehicle (n = 10) | Sham + PLX5622 (n = 10) | p-value | |
|---|---|---|---|
| BW WPI 0 (ff) | 3.4 ± 1.5 | 1.7 ± 0.9 | >0.999a |
| BW WPI 1 (ff) | 2.8 ± 0.9 | 5.7 ± 2.9 | 0.9937a |
| BW WPI 2 (ff) | 5.2 ± 2.1 | 8.7 ± 2.4 | 0.9677a |
| BW WPI 3 (ff) | 2.0 ± 1.2 | 5.2 ± 2.3 | 0.9822a |
| BW WPI 4 (ff) | 5.6 ± 1.5 | 8.7 ± 2.4 | 0.9884a |
| BW WPI 5 (ff) | 4.3 ± 1.5 | 10.8 ± 1.4 | 0.2911a |
| BW WPI 6 (ff) | 5.9 ± 2.7 | 6.6 ± 1.9 | >0.999a |
| BW WPI 7 (ff) | 5.6 ± 2.1 | 9.6 ± 3.1 | 0.9151a |
| BW WPI 8 (ff) | 4.4 ± 2.3 | 11.6 ± 2.3 | 0.1688a |
| BW WPI 9 (ff) | 8.9 ± 1.9 | 12.2 ± 1.8 | 0.9770a |
| BW WPI 10 (ff) | 8.8 ± 1.2 | 11.2 ± 1.8 | 0.9988a |
| BW WPI 11 (ff) | 10.4 ± 2.8 | 10.9 ± 2.0 | >0.999a |
| BW WPI 12 (ff) | 10.2 ± 2.3 | 75.1 ± 4.4 | >0.999a |
| Y maze (% spontaneous alternation) | 71.1 ± 3.7 | 71.4 ± 3.0 | 0.9507b |
| NOR (% preference index) | 78.0 ± 6.4 | 68.3 ± 3.6 | 0.2532b |
| MWM acquisition day 1 (time to escape) | 73.9 ± 4.1 | 75.1 ± 4.4 | >0.999a |
| MWM acquisition day 2 (time to escape) | 46.1 ± 7.7 | 56.0 ± 6.9 | 0.996a |
| MWM acquisition day 3 (time to escape) | 56.4 ± 6.9 | 58.1 ± 4.9 | >0.999a |
| MWM acquisition day 4 (time to escape) | 48.6 ± 6.2 | 40.1 ± 5.6 | >0.999a |
| MWM probe test (time in escape quadrant) | 26.7 ± 4.1 | 23.2 ± 2.9 | 0.3218c |
Neurobehavioral testing confirmed that sham + vehicle and sham + PLX6522-treated C57BL/6J mice had equal performance in BW, Y-maze, NOR, and MWM tests. Summary statistical data and p-values are shown for each neurobehavioral test (n = 10/group).
WPI, weeks postinjury; ff, foot faults.
aSidak's multiple-comparisons test.
bMann–Whitney test.
cUnpaired t test.