Table 1.
Proteins immunoprecipitated with GABABRs from cultured cortical neurons
| Protein ID | Protein name | IgG, % coverage | R1, % coverage |
|---|---|---|---|
| USMG5 | Up-regulated during skeletal muscle growth protein | 0 | 25.9 |
| RL39 | 60S ribosomal protein L39 | 0 | 19.6 |
| RL37A | 60S ribosomal protein L37a | 0 | 14.7 |
| CNRP1 | CB1 cannabinoid receptor-interacting protein 1 | 0 | 9.8 |
| PROF1 | Profilin-1 OS = Mus musculus | 0 | 8.6 |
| GDIR1 | Rho GDP-dissociation inhibitor 1 | 0 | 7.8 |
| RS26 | 40S ribosomal protein S26 | 0 | 7.8 |
| RS15A | 40S ribosomal protein S15a | 0 | 6.9 |
| DPYL1 | Dihydropyrimidinase-related protein 1 | 0 | 7.25 |
| RL27A | 60S ribosomal protein L27a | 0 | 6.8 |
| H10 | Histone H1.0 | 0 | 6.7 |
| COF1 | Cofilin-1 OS= | 0 | 6.6 |
| KV5A5 | Ig kappa chain V-V region T1 | 0 | 8.95 |
| RAB2A | Ras-related protein Rab-2A | 0 | 6.1 |
| RAP2A | Ras-related protein Rap-2a | 0 | 6 |
| CANB1 | Calcineurin subunit B type 1 | 0 | 5.9 |
| OTUB1 | Ubiquitin thioesterase OTUB1 | 0 | 5.5 |
| RS16 | 40S ribosomal protein S16 | 0 | 5.5 |
| RL9 | 60S ribosomal protein L9 | 0 | 5.2 |
| RL11 | 60S ribosomal protein L11 | 0 | 6.5 |
| RAB5A | Ras-related protein Rab-5A | 0 | 5.1 |
| NDUS3 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial | 0 | 4.9 |
| TPM3 | Tropomyosin alpha-3 chain | 0 | 4.6 |
| PGAM1 | Phosphoglycerate mutase 1 | 0 | 5.7 |
| RL7 | 60S ribosomal protein L7 | 0 | 4.1 |
| HNRPD | Heterogeneous nuclear ribonucleoprotein D0 | 0 | 3.9 |
| PP2AA | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | 0 | 3.6 |
| ERLN1 | Erlin-1 | 0 | 3.5 |
| SC6A1 | Sodium- and chloride-dependent GABA transporter 1 | 0 | 3.5 |
| EF1G | Elongation factor 1-gamma | 0 | 3 |
| SFXN5 | Sideroflexin-5 | 0 | 2.9 |
| SERA | D-3-phosphoglycerate dehydrogenase | 0 | 2.8 |
| 2ABA | Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform | 0 | 2.7 |
| ROA3 | Heterogeneous nuclear ribonucleoprotein A3 | 0 | 2.6 |
| NDUAA | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial | 0 | 2.5 |
| GUAD | Guanine deaminase | 0 | 2.5 |
| Sep-05 | Septin-5 | 0 | 2.4 |
| NPTN | Neuroplastin | 0 | 2.65 |
| Sep-03 | Neuronal-specific septin-3 | 0 | 2.3 |
| ARP3 | Actin-related protein 3 | 0 | 2.2 |
| PFKAM | ATP-dependent 6-phosphofructokinase, muscle type | 0 | 1.6 |
| TCPG | T-complex protein 1 subunit gamma | 0 | 2 |
Shown are the results of mass spectrometry analysis of proteins immunoprecipitated with antibodies against GABABR1 or IgG from mouse cortical neurons. Samples were digested with trypsin and subjected to LC-MS/MS to identify proteins precipitated with GABABR1 antibodies. The percentage coverage of each target protein is shown. The experimental data represent mean of two samples analyzed in parallel (duplicates). Note detection of PP2A-Cα (PP2AA) and PP2A-B55α (2ABA) subunits.