Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Schmidtea mediterranea) |
Asexual clonal line CIW4 of Schmidtea mediterranea | PMID:12421706 | RRID:NCBITaxon:79327 | All animals used in this study |
| Recombinant DNA reagent | pBluescript II SK(+) (plasmid) | Agilent Technologies | Cat:212205 | For cloning from ESTs |
| Recombinant DNA reagent | pJC53.2 (plasmid) | PMID:20967238 | RRID:Addgene_26536 | For cloning |
| Antibody | (mouse, monoclonal) Muscle antibody 6G10 | doi:10.1186/s12861-014-0050-9 | Used at 1:2000 | |
| Chemical compound, drug | Formaldehyde | EMD Millipore | Cat:FX0410-5 | Used at 4% in 1xPBS |
| Chemical compound, drug | Platinium Taq | Invitrogen | Cat:10966026 | For PCR |
| Chemical compound, drug | Trizol | Invitrogen | Cat:15596026 | Used for RNA extraction |
| Chemical compound, drug | RNAseZAP | Invitrogen | Cat:AM9780 | For LCM |
| Chemical compound, drug | Mayer’s Hematoxylin | Sigma Aldrich | Cat:MHS16-500ML | For LCM |
| Chemical compound, drug | Alcoholic Eosin Y | Sigma Aldrich | Cat:HT110116-500ML | For LCM |
| Commercial assay or kit | RNA Screen Tape | Agilent | Cat:5067–5576 | Used to verify RNA quality |
| Commercial assay or kit |
PicoPure RNA Isolation Kit | Arcturus | Cat:12204–1 | For LCM |
| Commercial assay or kit |
Quantseq 3’ mRNA Library Prep Kit FWD |
Lexogen | Cat:K01596 | For RNA-seq |
| Commercial assay or kit | iScript Kit | Bio-Rad | Cat:1708891 | For cDNA synth |
| other | PEN membrane slides |
Leica | Cat:11505158 | For LCM |
| Software, algorithm | Bestus Bioinformaticus Duk | DOE Joint Genome Institute | RRID:SCR_016969 | RNAseq read trimming |
| Software, algorithm | FastQC | Babraham Institute | RRID:SCR_014583 | RNAseq quality check |
| Software, algorithm | Bowtie2 | DOI:10.1038/nmeth.1923 | RNAseq transcript mapping | |
| Software, algorithm | Samtools v1.3 | PMID:19505943 | RRID:SCR_002105 | RNAseq processing |
| Software, algorithm | edgeR v3.8.6 | PMID:19910308 | RRID:SCR_012802 | RNAseq differential expression |
| Software, algorithm | TBLASTX | U.S. National Library of Medicine | RRID:SCR_011823 | For Human gene comparison |
| Software, algorithm | BLASTX | U.S. National Library of Medicine | RRID:SCR_001653 | Homology searches |
| Software, algorithm | BiNGO | PMID:15972284 | RRID:SCR_005736 | Gene Ontology |
| Software, algorithm |
NCBI ORFinder | U.S. National Library of Medicine | RRID:SCR_016643 | For ORF identification |
| Software, algorithm | NCBI CD-Search | U.S. National Library of Medicine | For mucin domain search | |
| Software, algorithm | Pfam 31.0 | DOI:10.1093/nar/gkv1344 | RRID:SCR_004726 | For mucin domain search |
| Software, algorithm | SMART | DOI: 10.1093/nar/gkx922 |
RRID:SCR_005026 | For mucin domain search |
| Software, algorithm | Zen (version 11.0.3.190 2012-SP2) | Zeiss | RRID:SCR_013672 | For microscope images |
| Software, algorithm | ImageJ (1.51 k) | DOI:10.1038/nmeth.2089 | RRID:SCR_002285 | For area and length analysis |
| Software, algorithm | R Studio (1.2.1335) | RStudio, Inc | RRID:SCR_000432 | For bioinformatics |
| Software, algorithm | Prism (v8.3.0) | GraphPad | RRID:SCR_002798 | Graphing |