Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (human) | TAPBPRwt | PMID:29025996 | lumenal domain | |
| Gene (human) | TAPBPRΔSL | This study (Figure 1C, Materials and methods section) |
lumenal domain | |
| Gene (human) | TAPBPRTsn-SL | This study (Figure 1C, Materials and methods section) |
lumenal domain | |
| Gene (human) | TAPBPRTN3-Ala | PMID:19119025 | lumenal domain | |
| Gene (human) | TAPBPRTN6 | PMID:19119025 | lumenal domain | |
| Gene (human) | HLA-A*02:01 | This study (Materials and methods section) |
ectodomain | |
| Gene (human) | β2-microglobulin | PMID:29025996 | ||
| Gene (mouse) | H2-Db | PMID:29025996 | ectodomain | |
| Strain, strain background (Escherichia coli) | DH10Bac | Thermo Fisher Scientific | 10361012 | chemically competent |
| Strain, strain background (Escherichia coli) | BL21(DE3) | Sigma-Aldrich | CMC0014 | chemically competent |
| Recombinant DNA reagent | pET-22 | Novagen/ Merck Millipore |
69744 | vector for protein expression in E. coli |
| Recombinant DNA reagent | pET-28 | Novagen/ Merck Millipore |
69864 | vector for protein expression in E. coli |
| Recombinant DNA reagent |
pFastBacI-gp67 | PMID:29025996 | transfer vector for Bac-to-Bac system | |
| Cell line (Spodoptera frugiperda) | Sf9 | Thermo Fisher Scientific | 11496015 | |
| Cell line (Spodoptera frugiperda) | Sf21 | Thermo Fisher Scientific | 11497013 | |
| Peptide, recombinant protein | RGPGRAFJ*TI (photo-P18-I10) |
PMID:26869717 | J* denotes photo-cleavable amino acid | |
| Peptide, recombinant protein | ASNENMETM | IEDB: epitope ID 4602 | competitor peptide for H2-Db | |
| Peptide, recombinant protein | FLPSDEEPYV | This study (Materials and methods section) |
competitor peptide for HLA-A*02:01 | |
| Peptide, recombinant protein | TQSC*NTQSI | This study (Materials and methods section) |
C* denotes TAMRA-labeled Cys |
|
| Peptide, recombinant protein | FLPSDC*FPSF | This study (Materials and methods section) |
C* denotes TAMRA-labeled Cys |
|
| Peptide, recombinant protein | ASNC*NMETM | This study (Materials and methods section) |
C* denotes TAMRA-labeled Cys |
|
| Chemical compound, drug | TAMRA-5 maleimide | Thermo Fisher Scientific | T6027 | |
| Chemical compound, drug | TAMRA-6 C2 maleimide | Thermo Fisher Scientific | 48180 | |
| Chemical compound, drug | Fmoc-3-amino-3-(2-nitro)phenyl-propionic acid | Peptech | CAS #: 517905-93-0 |
|
| Software, algorithm | Prism 6 | GraphPad Software | ||
| Software, algorithm | Fityk 1.3.1 | DOI: 10.1107/S0021889810030499 |
||
| Other | Superdex 200 Increase 10/300 | GE Healthcare | 28990944 | SEC column |
| Other | Superdex 200 Increase 3.2/300 | GE Healthcare |
28990946 | SEC column |
| Other | Superdex 75 Increase 3.2/300 | GE Healthcare | 29148723 | SEC column |
| Other | HiLoad Superdex 75 16/60 |
GE Healthcare | 28989333 | SEC column |
| Other | Fluorolog-3 | Horiba Jobin Yvon | spectro-fluorometer | |
| Other | Äkta Purifier | GE Healthcare | protein purification | |
| Other | Agilent 1200 | Agilent | analytical SEC | |
| Other | Liberty Blue | CEM Corporation | peptide synthesizer | |
| Other | X-tremeGENE HP | Sigma-Aldrich | 6366236001 | transfection reagent |