TABLE 1.
Oligonucleotide primers used in this study
| Usage | Primer | Sequence (5′ to 3′) |
|---|---|---|
| PCR of the genomic DNA sequences | MrXbp-F | CGACCCCTTCCAACTCAAACGCAC |
| MrXbp-R | CTCCCACCCGTGCCACTTTTCCCC | |
| MrStuA-F | AAGACCCTCCTTCCGACCTCCTCT | |
| MrStuA-R | TATAACCCACAGCAAGTCGTCAAC | |
| Used as construction of gene disruption vector | MrXbpLF | CGGAATCAGTCAGCCCTCTCGGGTCCGTCATC |
| MrXbpLR | CCGCTCGAGCTGTGGGCAGATGCTTCGTGGTTCA | |
| MrXbpRF | GCTCTAGATGGAAGCGTTATCAGAGCAGAGCGA | |
| MrXbpRR | AACTGCAGGGCAAGTCACCACGAGCCCCGAGGG | |
| MrStLF | CGGAATTCGGCATTTCAAGTGAACACCAAGTCTT | |
| MrStLR | CCGCTCGAGATCATTTGGCAGATTGGACGACGGTA | |
| MrStRF | GCTCTAGATATCTCGCCACACATCTTTCAAGCAA | |
| MrStRR | CCCAAGCTTAGGCACAGGGTGGAATGTATCGGCTA | |
| Used as construction of gene complementation plasmid | MrXbp-HF | CCGCTCGAGCTCGGGCAAGGGCAAAGGCTCACA |
| MrXbp-HR | GCTCTAGACGATGCCTGGGTGAAGCGGTTCAAG | |
| MrSt-HF | GCTCTAGAGGAGTGTCCATCAATGTTCCGTAGT | |
| MrSt-HF | AACTGCAGAACAGGCGTGCTAAGTAACATAGAA | |
| Mutants by PCR verification | MrXbpF | CTCGGGCAAGGGCAAAGGCTCACA |
| MrXbpR | GCACCTCGCTGCCAATAATGAAGA | |
| MrXbp-OF | CCAGGCAGATAATAACAGCATTCA | |
| MrXbp-OR | TCGCTTTATTCGGATTCCTACTTC | |
| MrStR | TTGGGCAGTCTGACGGTTCAAGCC | |
| MrStF | TTGACCCAGAACTCTAAACCAGCA | |
| MrSt-OF | ATGGGAGGATGAAGGCAGTCTGTG | |
| MrSt-OR | CATTACCCATACCATTGGCTTCACT | |
| hph-F | GCTCTCGCTAAACTCCCCAATGTCA | |
| hph-R | CATTGACTGGAGCGAGGCGATGTTC | |
| RT-qPCR analysis | MrXbp-qF | GCATTCAATGTCTCGCAGTT |
| MrXbp-qR | ATTCGTGGGAATCTGAAAGG | |
| MrSt-qF | TCACTACGCTGGGTTCGATA | |
| MrSt-qR | TTCGACCTGAAAGCACAGAC | |
| Mrtef-qF | GTCATCGTCCTCAACCATC | |
| Mrtef-qR | CAGTCTCAACAGCCTTACC | |
| Mrtub-qF | GGCAAGGTCGCTATGAAG | |
| Mrtub-qR | CTGGATGGAGGTAGAGTTAC | |
| MrCat1-qF | GAGCCAGCCTCGTCTGTTCT | |
| MrCat1-qR | CTGGGCGAGGACGTTCTTCT | |
| MrCat2-qF | AAGGATCCGCAGAAGCGTGA | |
| MrCat2-qR | AAGGTGTGGCCTCCAGCAAT | |
| MrSod1-qF | TGCCTGCTTTATCAATCGAC | |
| MrSod1-qR | TCAAGAATGAGTGTGCGTCA | |
| MrSod2-qF | AGGTTCTCCCAGAAGAGGGA | |
| MrSod2-qR | CGTACGTGACGAACCTCAAC | |
| MrAp1-qF | ACATACGCCCAGAAAGAACC | |
| MrAp1-qR | AGTTGGTTGCAGCTCAAATG | |
| MrH101-qF | TGGAGAAGCGTTTGGAGGAT | |
| MrH101-qR | GCCACCAGAAGCATTGAGAG | |
| MrCp450-qF | CTATATGGCACGTCCACGAC | |
| MrCp450-qR | GCTACGATTCCTTGGTTGGT | |
| MrHog1-qF | GCATCGTGACTTGAAGCCTA | |
| MrHog1-qR | GGGCTCGGTAATATCGTGTT | |
| MrSlt2-qF | TGTGCGGCCTCAAGTATATC | |
| MrSlt2-qR | CGAGGCCAAAGTCACAGAT | |
| MrHapX-qF | AGCCACAGGAACTTCTCCAA | |
| MrHapX-qR | GACGCTCATGATGCTAGCTG | |
| MrPkac-qF | AGGAGGTTGGTGATGAAGGG | |
| MrPkac-qR | CCCAAGTATACGCGCTCAAG | |
| MrCwb-qF | GGTGATTTCTCGTGGCTGTC | |
| MrCwb-qR | GGCCTCTCGTCGAAATCTTG | |
| MrCel-qF | GAAATCTCGGCCACGTTCAA | |
| MrCel-qR | GGAGAGACTTGGACGCTGTA | |
| MrH90-qF | CCCAGAGACAAGCAACGAAG | |
| MrH90-qR | TCTCGCCCAAGTCTCTCATC | |
| MrPtp -qF | GCAGTTTCCGCTTCTCGATT | |
| MrPtp -qR | CTGTGATTGTCCAGCTCACG | |
| MrNsdD-qF | CAACGGAGCATATGGTCATC | |
| MrNsdD-qR | CATGCCTAGGTGAGACTGGA | |
| Mrlac-qF | AGTGCACCGCAAACAATCAT | |
| Mrlac-qR | GCGACTGTCTGGTTGAAGAC | |
| MrCcp-qF | GTCCTTGGTAGGGTTGACGA | |
| MrCcp-qR | ATGGTACTCTGAGCCTGCTG | |
| Mrpks-qF | GCCTCCTCCATCGTCAAGGT | |
| Mrpks-qR | GCGCTCGTCTCGAGGAAGTA | |
| MrAtp10-qF | TCTTCTGTCGGCTCTGGTTT | |
| MrAtp10-qR | AAGCTTCGAGTCTCCCACAA | |
| MrWsc-qF | CGGGCGATTTGACTCTTGTT | |
| MrWsc-qR | GTTGTGTTGTCGAAGGCAGT | |
| MrMas1-qF | ACGAACAATGCACACGTTCT | |
| MrMas1-qR | CTTCACTGGCTTGTCCATCG | |
| MrClp-qF | TCACTAGAACTTGCCTGCCA | |
| MrClp-qR | TTCTGGCTCTATGGCTACGG | |
| MrRad2-qF | CTGAGTGTCAGCGACTGTTG | |
| MrRad2-qR | TCCTTCCGCTGCTCACTAAA | |
| MrEmk1-qF | CGAGAATGTTGGAGGGCTTG | |
| MrEmk1-qR | TCGAGTGTCGAGGGTATTCG | |
| MrTapt1-qF | CGAGGTAGACCAGGACCATC | |
| MrTapt1-qR | TTCCATATCGTGCCTCGTGA | |
| MrMob-qF | CCTGACGTTACACCGTTGAC | |
| MrMob-qR | TAGGATGGTGCAGTTGAGCA | |
| MrSem-qF | GACTTGTTTCTCCCGGTTCG | |
| MrSem-qR | CTGACCAGCAACGAAGAAGG | |
| MrUce-qF | CGCTTCCCTACCGAATACC | |
| MrUce-qR | TCGTCGCCTGGTCTGTGGA | |
| MrSwi6-qF | CCACCCAGATTCTCAAGGTT | |
| MrSwi6-qR | TGCTCGCCTGTTTGTATCTC | |
| MrSok1-qF | GACCAACAACAACAGCCACT | |
| MrSok1-qR | TCTTGTCCGTCCCGTAACAA |