TABLE 2.
Levels of photoproducts in UV222-irradiated B. subtilis spores and growing cellsa
| Sample type | UV fluence (mJ/cm2) | No. of molecules/106 basesb
|
||||||
|---|---|---|---|---|---|---|---|---|
| TT-CPD | TT-6-4PP | TC-CPD | TC-6-4PP | CT-CPD | CC-CPD | SP | ||
| WT cells | 0 | ND | ND | ND | ND | ND | ND | ND |
| WT cells | 8.4 | 25 | 1.3 | ND | 4.5 | ND | ND | ND |
| WT cells | 17 | 64 | 1.2 | 2.2 | 5.2 | 2.3 | ND | ND |
| α− β− spores | 0 | ND | ND | ND | ND | ND | ND | ND |
| α− β− spores | 8.4 | 78 | ND | ND | ND | ND | ND | 831 |
| α− β− spores | 17 | 144 | 0.6 | ND | ND | ND | ND | 1,521 |
| WT spores | 0 | ND | ND | ND | ND | ND | ND | 102 |
| WT spores | 8.4 | 0.8 | 0.1 | 0.1 | ND | ND | ND | 10,878 |
| WT spores | 17 | 4.3 | 0.8 | 6.1 | 3 | 3.1 | 0.9 | 67,036 |
a
B. subtilis PS533 (wild-type [WT]) log-phase cells and PS533 and PS578 (α− β−) spores were or were not UV222 irradiated, DNA was isolated and hydrolyzed, and photoproducts were analyzed and quantitated as described in Materials and Methods.
b
ND, not detectable and <0.1 molecules/106 bases.