TABLE 2.
P. americana Bacteroidetes species-specific 16S rRNA primer sequences
| Taxon | Isolate | Primer direction | Primer sequence (5′→3′) | Tm (°C) | Amplicon length (bp) |
|---|---|---|---|---|---|
| Bacteroides | PAB224 | Forward | TCAAGAAGCCGAGCCGTAAG | 60.1 | 206 |
| Reverse | GCTGAGCTAATCCCCCGATAAG | 60.4 | 206 | ||
| PAB51 | Forward | CACGTGTGGAGTTTTGTATGT | 56.8 | 203 | |
| Reverse | ACCTCCACTATACTCAAGACGCC | 61.5 | 203 | ||
| PAB214 | Forward | ACGTGTAGTGTTTTGCATGTACCG | 62.1 | 210 | |
| Reverse | TCCGCCTACCTCTACTGTACTCA | 61.7 | 210 | ||
| PAB519 | Forward | TGGGAATAAAGTGACGTACGTGT | 60 | 231 | |
| Reverse | CGAATTCCGCCAACCTTTACTTTAC | 60.9 | 231 | ||
| Dysgonomonas | PAD25 | Forward | CTCCGAATGGGTACAAGGGTCAT | 62 | 218 |
| Reverse | TCCATGCAGAACCACTCGACTAG | 62 | 218 | ||
| PAD521 | Forward | CATTACGTGTAGTGTATTGCATGTACTG | 60.7 | 194 | |
| Reverse | CTCAAGGCTACCAGTTTCAACGG | 61.7 | 194 | ||
| PAD511 | Forward | TACGTGTAGTATATTGCATGTACCATATG | 59.3 | 209 | |
| Reverse | CCGCCTACTTCATCTATACTCAAGAAAC | 61.4 | 209 | ||
| PAD216 | Forward | GTACTAGGGTAAAACAGGGGACGT | 61.6 | 203 | |
| Reverse | AACCCAGTTTCAACGGCAATTTTAAG | 61.4 | 203 | ||
| PAD520 | Forward | GTGCTAGGGTAAAACATATCACGAGTG | 61.8 | 209 | |
| Reverse | AAGTCTTCCAGTTTCAACGGCAA | 61.2 | 209 | ||
| Parabacteroides | PAP52 | Forward | CTTCTTTTATTGGGGAATAACGGCAG | 60.7 | 220 |
| Reverse | TCAAGACTAACAGTTTCAACGGCA | 60.9 | 220 | ||
| Paludibacter | PAR221 | Forward | TTGTATGTACTTTACGAATAAGCATCGG | 60.1 | 190 |
| Reverse | GCCTCTACTGCACTCAAGAACAC | 61.4 | 190 |