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. 2020 Apr 1;86(8):e02911-19. doi: 10.1128/AEM.02911-19

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Copyright © 2020 Zhu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Complex sphingolipid improved osmotic tolerance. (A) The mRNA level of the complex sphingolipid biosynthesis genes in strains XCG002 and XCG010 at 0 M NaCl. (B) The mRNA level of complex sphingolipid biosynthesis genes in strains XCG002 and XCG010 at 1.0 M NaCl. (C) The ratio of complex sphingolipid content in strain XCG018 (deletion of LAC1 in strain XCG010) to that of the control strain XCG002 changed at 0 M and 1.0 M NaCl. (D) Strains XCG002, XCG010, and XCG018 were spotted on plates containing or not containing 1.0 M NaCl. (E) The survival rates of strains XCG002, XCG010, and XCG018 over a range of NaCl doses (0.00, 0.25, 0.50, 0.75, and 1.00 M). All data are presented as mean values from three independent experiments. Error bars indicate the standard deviations. *, P < 0.05; **, P < 0.01.