TABLE 2.

Primers used in this study

Primer function and name	Sequence (5′–3′)
Overexpression
FET4-F1	TAGTGGATCCATGGGTAAAATTGCA
FET4-F2	ATGACTCGAGCTATTTTTCCAACATCATA
ADH6-F1	CTAGTGGATCCATGTCTTATCCTGAGAA
ADH6-F2	ATGACTCGAGCTAGTCTGAAAATTCT
PHO89-F1	TAGTGGATCCATGGCTTTACATCAA
PHO89-F2	ATGACTCGAGTTATGTCATTTGGTATTC
EGT2-F1	GCAGGAATTCATGAATAAACTATTGTTACATCT
EGT2-F2	ATGACTCGAGTTACAGCAGAAATGAGA
SAH1-F1	TAGTGGATCCATGTCTGCTCCAGCT
SAH1-F2	ATGACTCGAGTCAATATCTGTAGTGG
HXT4-F1	TAGTGGATCCATGTCTGAAGAAGCTG
HXT4-F2	ATGACTCGAGCTACTTTTTTCCGAAC
SKG6-F1	TAGTGGATCCATGTACCACACCCATA
SKG6-F2	ATGACTCGAGTCAGTTGACGGTATAATT
ELO2-F1	TAGTGGATCCATGAATTCACTCGTTAC
ELO2-F2	ATGACTCGAGTTACCTTTTTCTTCTGTG
URA1-F1	TAGTGGATCCATGACAGCCAGTTTAACTA
URA1-F2	ATGACTCGAGTTAAATGCTGTTCAACTT
HXK2-F1	TAGTGGATCCATGGTTCATTTAGGTCC
HXK2-F2	ATGACTCGAGTTAAGCACCGATGATAC
YBL111C-F1	TAGTGGATCCATGAAAGTTTCCGATAG
YBL111C-F2	ATGACTCGAGTCAGTGACAAACTCCT
RNR1-F1	TAGTGGATCCATGTACGTTTATAAAAGAGAC
RNR1-F2	ATGACTCGAGTTAACCCGAACACATTTC
SRL1-F1	TAGTGGATCCATGCTTCAATCCGTT
SRL1-F2	ATGACTCGAGTCACCAACTGGTCGAA
Replace promoter
PADH1-F1	GCTGGAGCTCATCCTTTTGTTGTTTCC
PADH1-F2	GGATCCACTAGTTCTAGAAGTTGATTGTATGCTTGG
PTDH3-F1	GCTGGAGCTCTCATTATCAATACTGCCA
PTDH3-F2	CTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTC
Gene deletion
L-lac1-F1	GAATGAAAAATAGTTGGAAAGGAAACA
L-lac1-F2	AGCTCTTGTTTATTGATACTGTGTC
Leu2 (lac1)-F1	AGTATCAATAAACAAGAGCTATGTCTGCCCCTAAGAAGAT
Leu2 (lac1)-F2	CTTAAAAACACCGTTTTCCTTTAAGCAAGGATTTTCTTAACTTCTTCG
R-lac1-F1	AGGAAAACGGTGTTTTTAAGTAGTA
R-lac1-F2	CATATTTAGTTTGCACTGAAGGAGAA
RT-PCR
LAC1-F1	TTCACTTCTGGTAACACTA
LAC1-F2	CTAATAGCGAACGGTCTA
LAG1-F1	CTTGACTGGTGACTCTAA
LAG1-F2	TATGATATGGCTACGAACA
AUR1-F1	ATGGTCATACACTTCAAT
AUR1-F2	GGTTCATCAGTCATATTAAG
CSG2-F1	CAAGTGTAATAGGCTACG
CSG2-F2	AAGGTCAGATAGAAGGTTA
IPT1-F1	CATCTTCATTCACCGTAT
IPT1-F2	TTATTGCCATTGCTGTTA