FIG 1.

A vertical profile for heterotrophs (blue circles), sulfate reducers (SRB) (green circles), acidophilic iron oxidizers (aIOM) (red circles), and acidophilic sulfur-oxidizers (aSOM) (black circles) in solid samples taken from the NIT at the Kam Kotia Mine, determined by MPN. The data points represent means of triplicates for heterotrophs and SRB, and of five replicates for acidophiles. Dashed line indicates single measure of pH. MPN data are compared to percentages of total reads of SOM/IOM (blue bars), SOM (yellow bars), IOM (red bars), and SRB (green bars), as determined by high-throughput 16S rRNA amplicon sequencing of samples collected at site 1.