FIG 3.

Impact of T1SS-secreted adhesins and hilD expression on STM adhesion to corn salad. Corn salad grown under aseptic conditions was infected with STM strain SR11 Δ12 with the overexpression of the regulator hilD for analysis of the SPI4-encoded, T1SS-secreted adhesin SiiE and the SPI1-encoded T3SS (A). In addition, SR11 Δ12 expressing AHT-induced, T1SS-secreted wild-type adhesin BapA or the indicated BapA truncation mutants were tested (B). The adhesion and the statistical significances were determined as described in the legend to Fig. 1. (C) Schematic overview of truncated BapA forms used in adhesion assays.