TABLE 1.
Bacterial strains used in this study
| Strain | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli CC118 λpir | Cloning strain for λpir-dependent plasmids | 60 |
| E. coli DH5α MCR | Cloning strain | 61 |
| E. coli NEB5α | Cloning strain | New England Biolabs |
| E. coli S17-1 λpir | Mobilization strain for plasmids containing oriVR6K and mobRP4 | 62 |
| S. Typhimurium IR715 | Salmonella enterica serovar Typhimurium ATCC 14028s, spontaneous Nalr | 63 |
| S. Typhimurium LT2 | Wild type | 64 |
| S. Typhimurium NCTC12023 | Wild type | NCTC |
| S. Typhimurium SR11 | Wild type | 65 |
| AJB754 | IR715 ΔstiABCH::KSAC | 66 |
| AJB786 | IR715 ΔstbABCD::KSAC | 66 |
| EHW1 | IR715 ΔbcfABCDEFGH::KSAC | 66 |
| EHW2 | IR715 ΔfimAICDHF::KSAC | 66 |
| EHW3 | IR715 ΔstfACDEFG::KSAC | 66 |
| EHW11 | IR715 ΔstdAB::KSAC | 66 |
| SF22 | IR715 ΔsthABCDE::KSAC | 66 |
| SPN191 | IR715 ΔbcfABCDEFGH | This study |
| SPN192 | IR715 ΔfimAICDHF | This study |
| SPN193 | IR715 ΔlpfABCDE::KSAC | This study |
| SPN195 | IR715 ΔsafABCD::KSAC | This study |
| SPN196 | IR715 ΔstbABCD | This study |
| SPN198 | IR715 ΔstdAB | This study |
| SPN199 | IR715 ΔstfACDEFG | This study |
| SPN200 | IR715 ΔsthABCDE | This study |
| SPN201 | IR715 ΔstiABCH | This study |
| SPN202 | IR715 ΔstjEDCBA::KSAC | This study |
| SPN226 | IR715 ΔbcfABCDEFGH::pSF1 | This study |
| SPN227 | IR715 ΔfimAICDHF::pSPN22 | This study |
| SPN230 | IR715 ΔsafABCD | This study |
| SPN231 | IR715 ΔstbABCD::pSF38 | This study |
| SPN233 | IR715 ΔstdAB::pSPN3 | This study |
| SPN234 | IR715 ΔstfACDEFG::pSF5 | This study |
| SPN235 | IR715 ΔsthABCDE::pSF25 | This study |
| SPN236 | IR715 ΔstiABCH::pSPN2 | This study |
| SPN237 | IR715 ΔstjEDCBA | This study |
| SPN251 | IR715 ΔsafABCD::pSPN13 | This study |
| SPN252 | IR715 ΔstjEDCBA::pSPN14 | This study |
| SPN334 | IR715 ΔpefACD-orf5-orf6::KSAC | This study |
| SPN335 | IR715 ΔpefACD-orf5-orf6 | This study |
| SPN336 | IR715 ΔpefACD-orf5-orf6::pSPN16 | This study |
| SPN337 | IR715 ΔstcABCD::KSAC | This study |
| SPN338 | IR715 ΔstcABCD | This study |
| SPN339 | IR715 ΔstcABCD::pSPN15 | This study |
| SPN365 | SR11 ΔfimAICDHF | This study |
| SPN366 | SR11 ΔfimAICDHF ΔstbABCD | This study |
| SPN367 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE | This study |
| SPN368 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG | This study |
| SPN369 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG ΔstiABCH | This study |
| SPN370 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG ΔstiABCH ΔbcfABCDEFGH | This study |
| SPN371 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG ΔstiABCH ΔbcfABCDEFGH ΔsafABCD | This study |
| SPN372 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG ΔstiABCH ΔbcfABCDEFGH ΔsafABCD ΔpefACD-orf5-orf6 | This study |
| SPN373 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG ΔstiABCH ΔbcfABCDEFGH ΔsafABCD ΔpefACD-orf5-orf6 ΔstcABCD | This study |
| SPN374 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG ΔstiABCH ΔbcfABCDEFGH ΔsafABCD ΔpefACD-orf5-orf6 ΔstcABCD ΔstjEDCBA | This study |
| SPN375 | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG ΔstiABCH ΔbcfABCDEFGH ΔsafABCD ΔpefACD-orf5-orf6 ΔstcABCD ΔstjEDCBA ΔstdAB | This study |
| SPN376 (=SR11 Δ12) | SR11 ΔfimAICDHF ΔstbABCD ΔsthABCDE ΔstfACDEFG ΔstiABCH ΔbcfABCDEFGH ΔsafABCD ΔpefACD-orf5-orf6 ΔstcABCD ΔstjEDCBA ΔstdAB ΔlpfABCDE::KSAC | This study |
| MvP493 | ΔSPI4::aph | 15 |
| MvP681 | ΔsadA::aph | 67 |
| MvP702 | Δwzz::aph | 46 |
| MvP703 | ΔfepE::aph | 46 |
| MvP813 | ΔinvC::aph | 68 |
| MvP886 | ΔrfaL::aph | 69 |
| MvP1208 | ΔsopB::aph | 70 |
| MvP1209 | ΔcheY::aph | 71 |
| MvP1210 | ΔfliI::aph | 71 |
| MvP1412 | ΔsopE2::aph | 18 |
| MvP1472 | ΔsopA::aph | This study, construction intermediate |
| MvP1527 | ΔcheZ::aph | 71 |
| MvP1611 | ΔbapABCD::aph | This study, construction intermediate |
| MvP1663 | ΔsadA::aph | This study, construction intermediate |
| MvP1754 | ΔfliC::aph | 72 |
| MvP1755 | ΔfljB::aph | 72 |
| MvP1760 | ΔfliC ΔfljB::aph | 72 |
| MvP1825 | ΔshdA::aph | This study |
| MvP1827 | ΔmisL::aph | This study |
| MvP1842 | PtetA::shdA | This study, construction intermediate |
| MvP1884 | ΔsipA::aph | 18 |
| MvP1885 | ΔsopD::aph | This study, construction intermediate |
| MvP2050 | ΔmotAB::aph | 73 |
| MvP2447 | Δ12 ΔmisL | This study |
| MvP2448 | Δ12 ΔmisL ΔshdA::aph | This study, construction intermediate |
| MvP2449 | Δ12 ΔmisL ΔshdA | This study, construction intermediate |
| MvP2456 | Δ12 ΔmisL ΔshdA ΔSPI4::aph | This study, construction intermediate |
| MvP2457 | Δ12 ΔmisL ΔshdA ΔSPI4 | This study, construction intermediate |
| MvP2458 | Δ12 ΔmisL ΔshdA ΔSPI4 ΔbapABCD::aph | This study, construction intermediate |
| MvP2486 | Δ12 ΔmisL ΔshdA ΔSPI4 ΔbapABCD | This study, construction intermediate |
| MvP2487 | Δ12 ΔmisL ΔshdA ΔSPI4 ΔbapABCD ΔsadA::aph | This study, construction intermediate |
| MvP2488 | Δ12 ΔmisL ΔshdA ΔSPI4 ΔbapABCD ΔsadA | This study, construction intermediate |
| MvP2506 | Δ12 rck::aph-I-SceI | This study |
| MvP2507 | Δ12 pagN::aph-I-SceI | This study |
| MvP2508 | Δrck::aph-I-SceI | This study |
| MvP2509 | ΔpagN::aph-I-SceI | This study |
| MvP2518 | Δ12 ΔmisL ΔshdA ΔSPI4 ΔbapABCD ΔsadA Δrck::aph-I-SceI | This study, construction intermediate |
| MvP2535 | Δ12 ΔmisL ΔshdA ΔSPI4 ΔbapABCD ΔsadA Δrck | This study, construction intermediate |
| MvP2533 | Δ12 ΔmisL ΔshdA ΔSPI4 ΔbapABCD ΔsadA Δrck ΔpagN::aph-I-SceI | This study, construction intermediate |
| MvP2537 | Δ12 ΔmisL ΔshdA ΔSPI4 ΔbapABCD ΔsadA Δrck ΔpagN | This study |
| MvP2622 | Δ12 shdA::aph | This study |
| MvP2623 | Δ12 sadA::aph | This study |
| MvP2624 | Δ12 SPI4::aph | This study |
| MvP2625 | Δ12 bapABCD::aph | This study |
| MvP2702 | ΔcsgBAC-DEFG::aph | This study, construction intermediate |
| MvP2703 | Δ12 csgBAC-DEFG::aph | This study |
| MvP2706 | Δ12 ΔmisL ΔsadA ΔshdA ΔSPI4 ΔbapABCD Δrck ΔpagN ΔcsgBAC-DEFG::aph | This study, construction intermediate |
| MvP2707 (=SR11 Δ20) | Δ12 ΔmisL ΔsadA ΔshdA ΔSPI4 ΔbapABCD Δrck ΔpagN ΔcsgBAC-DEFG | This study |
| MvP2710 | Δ12 ΔmisL ΔsadA ΔshdA ΔSPI4 ΔbapABCD Δrck ΔpagN ΔcsgBAC-DEFG ΔfliI::aph | This study |
| MvP2711 | Δ12 ΔmisL ΔsadA ΔshdA ΔSPI4 ΔbapABCD Δrck ΔpagN ΔcsgBAC-DEFG ΔmotAB::aph | This study |
| MvP2718 | Δ12 ΔinvC::aph | This study |
| MvP2788 | Δ12 ΔfepE::aph | This study, construction intermediate |
| MvP2789 | Δ12 Δwzz::aph | This study, construction intermediate |
| MvP2790 | Δ12 ΔrfaL::aph | This study, construction intermediate |
| MvP2798 | Δ12 ΔfepE | This study |
| MvP2799 | Δ12 Δwzz | This study |
| MvP2800 | Δ12 ΔrfaL | This study |
| MvP2812 | Δ12 ΔfepE Δwzz::aph | This study |
| MvP2819 | Δ12 ΔsopB::aph | This study, construction intermediate |
| MvP2828 | Δ12 ΔsopB | This study, construction intermediate |
| MvP2829 | Δ12 ΔsopB ΔsopA::aph | This study, construction intermediate |
| MvP2831 | Δ12 ΔsopB ΔsopA | This study, construction intermediate |
| MvP2832 | Δ12 ΔsopB ΔsopA ΔsopE2::aph | This study, construction intermediate |
| MvP2835 | Δ12 ΔsopB ΔsopA ΔsopE2 | This study, construction intermediate |
| MvP2841 | Δ12 ΔsopB ΔsopA ΔsopE2 ΔsopD::aph | This study, construction intermediate |
| MvP2843 | Δ12 ΔsopB ΔsopA ΔsopE2 ΔsopD | This study, construction intermediate |
| MvP2844 (=SR11 Δeffector proteins) | Δ12 ΔsopB ΔsopA ΔsopE2 ΔsopD ΔsipA::aph | This study |
| MvP2864 | Δ12 ΔmisL::aph | This study |