TABLE 2.
Plasmids used in this study
| Plasmid | Relevant genotype | Reference or source |
|---|---|---|
| pE-FLP | FLP recombinase expression | 74 |
| pKD4 | aph resistance cassette flanked by FRT sites; Kmr Carbr | 58 |
| pKD13 | aph resistance cassette flanked by FRT sites, temp-sensitive replication (30°C); Kmr Carbr | 58 |
| pWRG730 | Red recombinase expression | 59 |
| p3313 | pWSK29 rfaDFCL | 69 |
| p3773 | tetR PtetA | 31 |
| p4253 | tetR PtetA::bapABCD in pWSK29 | 31 |
| p4318 | tetR PtetA::bapA[ΔBIg1]BCD in pWSK29 | This study |
| p4321 | tetR PtetA::bapA[ΔBIg28]BCD in pWSK29 | This study |
| p4320 | tetR PtetA::bapA[ΔBIg15-24]BCD in pWSK29 | This study |
| p4331 | tetR PtetA::bapA[ΔBIg1-28]BCD in pWSK29 | This study |
| p4380 | tetR PtetA::csgBACEFG in pWSK29 | 31 |
| p4389 | tetR PtetA::stiABCD in pWSK29 | 31 |
| p4390 | tetR PtetA::stfABCDEFG in pWSK29 | 31 |
| p4391 | tetR PtetA::stbABCDEFG in pWSK29 | 31 |
| p4392 | tetR PtetA::fimAICDHF in pWSK29 | 31 |
| p4393 | tetR PtetA::safABCD in pWSK29 | 31 |
| p4394 | tetR PtetA::stdABCD in pWSK29 | 31 |
| p4395 | tetR PtetA::stjABCDE in pWSK29 | 31 |
| p4396 | tetR PtetA::pefACDEF in pWSK29 | 31 |
| p4397 | tetR PtetA::bcfABCDEFG in pWSK29 | 31 |
| p4399 | tetR PtetA::stcABC in pWSK29 | 31 |
| p4400 | tetR PtetA::sthABCDE in pWSK29 | 31 |
| p4401 | tetR PtetA::pagN in pWSK29 | 31 |
| p4402 | tetR PtetA::rck in pWSK29 | 31 |
| p4403 | tetR PtetA::misL in pWSK29 | 31 |
| p4519 | tetR PtetA::lpfABCDE in pWSK29 | 31 |
| p4520 | tetR PtetA::shdA in pWSK29 | 31 |
| p4904 | tetR PtetA::hilD in pWSK29 | This study |
| p5035 | tetR PtetA::sadBA in pWSK29 | This study |
| pBluescriptII KS+ | Cloning vector; Carbr | 75 |
| pBS34 | pBluescriptII KS+ [XbaI][PstI]KSAC[PstI][XbaI]); Carbr Kmr | 76 |
| pCR2.1 | TOPO TA cloning vector; Carbr Kmr | Invitrogen |
| pEW5 | pRDH10 ΔstdAB (−60 to +3219)::KSAC; Cmr Tetr Kmr | 12 |
| pEW13 | pRDH10 ΔstiABCH (+40 to +4992)::KSAC); Cmr Kmr | 12 |
| pRDH10 | oriVR6K sacRB mobRP4; Cmr Tetr | 77 |
| pSF1 | pRDH10 ΔbcfABCDEFGH (+47 to +6830); Cmr Tetr | 12 |
| pSF2 | pRDH10 ΔfimAICDHF (+40 to +5970); Cmr Tetr | 66 |
| pSF5 | pRDH10 ΔstfACDEFG (−122 to +5493); Cmr Tetr | 12 |
| pSF25 | pRDH10 ΔsthABCDE (−6 to +5420); Cmr Tetr | 12 |
| pSF38 | pRDH10 ΔstbABCD (−59 to +5183); Cmr | 12 |
| pSPN2 | pEW13 ΔstiABCH (+40 to +4992); Cmr | This study |
| pSPN3 | pEW5 ΔstdAB (−60 to +3219); Cmr Tetr | This study |
| pSPN5 | pCR2.1 (LPF-FR1); Carbr Kmr | This study |
| pSPN6 | pCR2.1 ΔsafABCD (−45 to +4364); Carbr Kmr | This study |
| pSPN7 | pCR2.1 ΔstjEDCBA (−49 to +5185); Carbr Kmr | This study |
| pSPN8 | pCR2.1 ΔstcABCD (−65 to +4827); Carbr Kmr | This study |
| pSPN9 | pCR2.1 ΔpefACD-orf5-orf6 (−110 to +5610); Carbr Kmr | This study |
| pSPN12 | pCR2.1 (LPF-FR2); Carbr Kmr | This study |
| pSPN13 | pRDH10 ΔsafABCD (−45 to +4364); Cmr | This study |
| pSPN14 | pRDH10 ΔstjEDCBA (−49 to +5185); Cmr | This study |
| pSPN15 | pRDH10 ΔstcABCD (−65 to +4827); Cmr | This study |
| pSPN16 | pRDH10 ΔpefACD-orf5-orf6 (−110 to +5610); Cmr | This study |
| pSPN17 | pBluescriptII KS+ ([BamHI]LPF-FR1[PstI]); Carbr | This study |
| pSPN18 | pSPN13 ΔsafABCD (−45 to +4364)::KSAC); Cmr Kmr | This study |
| pSPN19 | pSPN14 ΔstjEDCBA (−49 to +5185)::KSAC); Cmr Kmr | This study |
| pSPN20 | pSPN15 ΔstcABCD (−65 to +4827)::KSAC); Cmr Kmr | This study |
| pSPN21 | pSPN16 ΔpefACD-orf5-orf6 (−110 to +5610)::KSAC); Cmr Kmr | This study |
| pSPN22 | pRDH10 ΔfimAICDHF (+40 to +5970); Cmr | This study |
| pSPN26 | pSPN17 ([BamHI]LPF-FR1[PstI]LPF-FR2[Acc65I]); Carbr | This study |
| pSPN27 | pRDH10 ΔlpfABCDE (−60 to +5325); Cmr | This study |
| pSPN37 | pSPN27 ΔlpfABCDE (−60 to +5325)::KSAC); Cmr Kmr | This study |