TABLE 3.
Strains used in this study
| Strain | Characteristics | MIC of nisin A (μg/ml) | Reference or source |
|---|---|---|---|
| S. aureus | |||
| MW2 | Clinical strain, methicillin-resistant (mecA+) | 512 | 45 |
| COL | Clinical strain, methicillin-resistant (mecA+) | 1,024 | 46 |
| TY34 | Clinical strain, methicillin-resistant (mecA+) | 512 | 47 |
| MM2202 | pYT1 insertion in MW1875 (MW1875 to MW1871 inactivation) in MW2, TETr | 512 | This study |
| MM2280 | Overexpression of MW1875 to MW1871 (pCL8) in MW2, CHLr | 2,048 | This study |
| MM2259 | pYT1 insertion in MW1875 (MW1874 to MW1871 overexpression) in MW2, TETr | 2,048 | This study |
| SAN2 | Nisin A-resistant mutant from MW2 | 2,048 | This study |
| MM2278 | pYT1 insertion in MW1875 (MW1875 to MW1871 inactivation) in SAN2, TETr | 512 | This study |
| MM2153 | pYT1 insertion in MW1874 (MW1874 to MW1871 inactivation) in SAN2, TETr | 512 | This study |
| MM2279 | MW1875 to MW1871 (SAN2) complementation in MM2278, TETr, CHLr | 2,048 | This study |
| SAN469 | Nisin A-resistant mutant from MW2 | 2,048 | This study |
| SAN233 | Nisin A-resistant mutant from COL | 2,048 | This study |
| SAN455 | Nisin A-resistant mutant from TY34 | 2,048 | This study |
| E. coli | |||
| XL-II | endA1 supE44 thi-1 hsdR17 recA1 gyrA96 relA1 lac [F′ proAB lacIqZ∆M15 Tn10 (TETr) Amy CHLr] | Stratagene | |
| MM1127 | His tag-fused MW1875 (MW2) gene in XL-II, AMPr | This study | |
| MM1128 | His tag-fused MW1875 (SAN2) gene in XL-II, AMPr | This study |