TABLE 4.

Primers used in this study^a

Target gene ID	Primer-forward	Primer-reverse
Construction of gene-inactivated mutants
pmtR + pmtA-D	5′-TTGGATCCACAATAGTGATTTTCCGATT-3′	5′-CAAAGCTTTGGCTTGCGCTTCATTAA-3′
pmtA-D	5′-AGGGATCCATATGGCTCTCAATCCG-3′	5′-AAAAGCTTCACTCACAACTTGATATC-3′
Construction of the plasmid for gene complementation
pmtR + pmtA-D-pCL15	5′-ATAAGCTTATACAGAAAGTGATAGGG-3′	5′-TTGGATCCAACTGATCACTTGAATAATT-3′
pmtA-D-pCL15	5′-ATAAGCTTTTTTAAGCTTCATTTATGAG-3′	5′-TTGGATCCAACTGATCACTTGAATAATT-3′
pmtR + pmtA-D-pCL8	5′-CTAAGCTTTTGAAGTAGACAATGCAAG-3′	5′-ATCCCGGGTTCCCAACCTCAAAATTAT-3′
Pmt promoter + pmtA-D-pYT1	5′-CAAGATCTAATGGTAGTGTCATTTCATT-3′	5′-CAGGATCCCAACGTCCCCCTATCAC-3′
	5′-TTGGATCCACAATAGTGATTTTCCGATT-3′	5′-CAAAGCTTTGGCTTGCGCTTCATTAA-3′
Amplification of DNA fragments used in gel shift assay
pmtR-F1	5′-AATGGTAGTGTCATTTCATTT-3′	5′-CAACGTCCCCCTATCAC-3′
Construction of the plasmid for recombinant protein
rPmtR	5′-CCGGATCCATGAAAATAATTTTAAAAAACAAT-3′	5′-TTAACGTTTCATGATGATTCCTCCTCA-3′
rPmtA	5′-CCGGATCCATGAATGCCATAGAATTAAG-3’	5′-TTAAGCTTTTAAAAACCTTCTTCCATCA-3′
Primers for quantitative PCR
pmtR	5′-AATTGGTTAATGAAGCGCAAG-3′	5′-GATTCCTCCTCATAAATGAACG-3′
pmtA	5′-TAAAGCTTCGTTCATTTATGAGGAGG-3′	5′-ACGATAAAAAGGGGCAATCA-3′
vraD	5′-CACTTGCCAAATTCCGTA-3′	5′-AATACCTAATGCTGTCGTGA-3′
gyrB	5′-AGGTCTTGGAGAAATGAATG-3′	5′-CAAATGTTTGGTCCGGTT-3′
Primers used for the DNA sequence
pmtR, A-D-seq-F-500	5′-TGAAATTCAATAACTTATTAAA-3′
pmtR, A-D-F-26	5′-ATACAGAAAGTGATAGGG-3′
pmtR, A-D-F351	5′-AACGTTCATTTATGAGGAGG-3′
pmtR, A-D-F967	5′-ATTATATCATTCACTTAAGTG-3′
pmtR, A-D-F1429	5′-TTTGGATTTTAGATGCTGGTCA-3′
pmtR, A-D-F1967	5′-ATTACAAAAAAATACGGCTC-3′
pmtR, A-D-F2409	5′-ATGGCTCAATTGATGTGCTG-3′
pmtR, A-D-F3047	5′-CGCGTGATTTTTCACAAGGT-3′
pmtR, A-D-seq-R		5′-TTTAAAATTCCCAACCTCA-3′

^aUnderlines indicate the recognition site of the respective restriction enzyme.