TABLE 3.
Oligonucleotide primers used for creation of deletion constructs and plasmids and assessment of sequence integrity, integration, and excision
| Primer | Sequence 5′–3′a |
|---|---|
| 241Eco_F | GATGAATTCACGCTGCTTAG |
| 241splice243_R | CGGCTTTTTGTCATATACTTTAACAGTCTTTCTTAT |
| 243Spe_R | CTACTAGTCATGATTGATTTTGGT |
| 243splice241_F | AGAAAGACTGTTAAAGTATATGACAAAAAGCCGA |
| 241_IF | GCTTCTACGTCACCAGCTTCT |
| 243_IR | TCCACAGTTTCGAACTGGTG |
| 240_F | ATGTCTAAAGTGTGACTATATGTT |
| 240splice242_R | TACTTTAACAGTCTTTCTTAGGCTTATTTTCCCTTCT |
| 242splice240_F | AGAAGGGAAAATAAGCCTAAGAAAGACTGTTAAAGTA |
| 242Spe_R | CATTTGACTAGTCATCATTCGGTAGTC |
| 240_IF | GAATGTCTAAAGTGTGACTATATGTT |
| 242_IR | ACGGTTGTATTCAGGCATATTC |
| pGhost1 | AGTCACGACGTTGTAAAACGACG |
| pGhostR | TACTACTGACAGCTTCCAAGG |
| pForVec | ACAGCAATGTTACAAGTTGAAAT |
| p181 | GCGAAGATAACAGTGACTCTA |
| 242_COD2F | AAAAAATTATCAATTATAGTTCCTTG |
| 242_C_R | GAAGCTCCACGTGAACTTC |
| 241_NdeF | TAACATATGGGTATTTTTAAAAGAATAC |
| 241_BamR | TTTGGATCCTTTAACAGTCTTTCTTATTAC |
a
Mismatching base pairs to insert restriction sites or for splice overlap extension are in bold.