FIG 3.
A. tumefaciens cells harboring pBBR1MCS2-Pphac-dsred2EC-lactC2 after growth on LB medium for 24 h. From left to right are bright-field, dsRed channel, DAPI-polyP channel, and merged images. The dsRed2EC-LactC2 protein formed a cap-like fluorescence signal at one cell pole (white arrow head), whereas the DAPI-polyP-specific foci (arrows) appeared at both poles. The overlay of the dsRed2EC and DAPI-polyP signals showed no colocalization. The experiment was performed in three biological replicates. A typical result is shown.