TABLE 4.
Primer and probe sequences of qPCR assay human-associated DNA marker and general fecal sewage metrics
| Assay | Primer/probe | Sequence (5′ to 3′)a | Reference(s) |
|---|---|---|---|
| CPQ_056 | crAss056_F1 | CAGAAGTACAAACTCCTAAAAAACGTAGAG | 27 |
| crAss056_R2 | GATGACCAATAAACAAGCCATTAGC | ||
| crAss056_P1 | FAM-AATAACGATTTACGTGATGTAAC-MGB | ||
| CPQ_064 | crAss064_F1 | TGTATAGATGCTGCTGCAACTGTACTC | |
| crAss064_R1 | CGTTGTTTTCATCTTTATCTTGTCCAT | ||
| crAss064_P1 | FAM-CTGAAATTGTTCATAAGCAA-MGB | ||
| HPyV | SM2 | AGTCTTTAGGGTCTTCTACCTTT | 21, 41 |
| P6 | GGTGCCAACCTATGGAACAG | ||
| KGj3 | FAM-TCATCACTGGCAAACAT-MGB | ||
| HF183/BacR287 | HF183 | ATCATGAGTTCACATGTCCG | 28 |
| BacR287 | CTTCCTCTCAGAACCCCTATCC | ||
| BacP234 | FAM-CTAATGGAACGCATCCC-MGB | ||
| Bac234IAC | VIC-AACACGCCGTTGCTACA-MGB | ||
| HumM2 | HumM2F | CGTCAGGTTTGTTTCGGTATTG | 26, 40 |
| HumM2R | TCATCACGTAACTTATTTATATGCATTAGC | ||
| HumM2P | FAM-TATCGAAAATCTCACGGATTAACTCTTGTGTACGC-TAMRA | ||
| UC1P1 | VIC-CCTGCCGTCTCGTGCTCCTCA-TAMRA | ||
| Entero1a | EnteroF1A | GAGAAATTCCAAACGAACTTG | 11, 12 |
| EnteroR1 | CAGTGCTCTACCTCCATCATT | ||
| GPL813TQ | FAM-TGGTTCTCTCCGAAATAGCTTTAGGGCTA-TAMRA | ||
| EC23S857 | EC23SF2-1 | GGTAGAGCACTGTTTTGGCA: | 10 |
| EC23SR2-1 | TGTCTCCCGTGATAACTTTCTC | ||
| EC23SP2b | FAM-TCATCCCGACTTACCAACCCG-TAMRA | ||
| GenBac3 | GenBactF3 | GGGGTTCTGAGAGGAAGGT | 51 |
| GenBactR4 | CCGTCATCCTTCACGCTACT | ||
| GenBactP2 | FAM-CAATATTCCTCACTGCTGCCTCCCGTA-TAMRA |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.