TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli strain | ||
| DH5α | F− Φ80lacZΔM15 Δ(lacZYA-argF)U169 rec1A end1A hsdR17 gyrA96 supE44 thi-1 relA1 | 43 |
| L. lactis strains | ||
| MG1363 | L. lactis subsp. cremoris plasmid-free derivative of NCDO712 | 44 |
| MGΔpepV | MG1363 carrying a chromosomal deletion of pepV | This study |
| MGΔpepVcodYR218C | MGΔpepV with mutation in CodY 218 residue from arginine to cysteine | This study |
| Plasmids | ||
| pCS1966 | Integration vector for L. lactis | 34 |
| pCS1966-pepV | pCS1966 containing pepV deletion construct | This study |