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. 2020 Feb 10;39(14):3028–3040. doi: 10.1038/s41388-020-1202-y

Fig. 3. Impact of IBL-302 on PIM/AKT/PI3K signalling components and induction of apoptosis in breast cancer cells.

Fig. 3

a SKBR-3 cells were treated with DMSO and IBL-302 (1 µM) for 3 h. The resulting total AKT, pAKT-(S473), pAKT-(T308), total mTOR, pmTOR-(S2448), pmTOR-(2481) and pBAD-(S138). b–g BT-474 and HCC-1954 cells were treated with ascending doses of BKM120 and IBL-302 (0.1, 0.3 and 1 µM) for 3, 8 and 24 h. The resulting pAKT-(S473) and pBAD-(S112) were analysed via western blot analysis. h, i BT-474 and HCC-1954 cells were treated with increasing doses of BKM120 and IBL-302 (0–10 µM) for 3 h. The resulting caspase 3/7 induction was analysed via caspase assay.