Table 1.
Linearity and repeatability of IPA extraction methodology. Human lymphoma cell line HL60 was cultured in RPMI 10% FCS and 1% PS. Linearity was assessed by calculating the correlation coefficient (Corr) using different numbers of cells (1.25, 2.5, 5.0, and 10.0 × 106). For repeatability, coefficient of variation (%CV) was used as a parameter, and was evaluated on four replicates (5.0 × 106). See also ESM S6
| Cell # × 106 | CE | CER | DAG | DCER | FFA | HCER | LCER | LPC | LPE | PC | PE | SM | TAG |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1.25 | 7.6 | 1.1 | 3.9 | 0.6 | 29.2 | 1.1 | 3.6 | 0.4 | 0.2 | 214.2 | 121.7 | 89.7 | 11.3 |
| 2.5 | 13.4 | 2.1 | 6.7 | 0.8 | 27.1 | 2.1 | 6.9 | 1.0 | 0.5 | 427.5 | 245.9 | 158.2 | 20.2 |
| 5 | 23.1 | 4.3 | 13.7 | 1.1 | 25.4 | 4.1 | 14.1 | 1.9 | 0.9 | 842.2 | 457.3 | 282.1 | 40.9 |
| 10 | 32.9 | 7.3 | 23.5 | 0.7 | 65.8 | 7.2 | 24.1 | 7.0 | 1.7 | 1401.5 | 413.7 | 417.0 | 72.1 |
| 25 | 120.4 | 8.8 | 90.0 | 3.8 | 91.9 | 22.5 | 49.5 | 13.8 | 3.8 | 3034.0 | 1385.2 | 681.8 | 114.1 |
| 50 | 243.5 | 15.3 | ND | ND | 135.2 | 43.9 | ND | 25.9 | 8.3 | 5617.7 | 2394.2 | 1262.3 | 217.5 |
| Blank | 0.5 | 0.05 | ND | 3,1 | 22.9 | ND | ND | ND | ND | ND | ND | 3.3 | 0.8 |
| Coeff. corr | 1.00 | 0.93 | 0.98 | 0.87 | 0.93a | 1.00 | 0.99 | 0.99 | 1.00 | 1.00 | 0.99 | 0.99 | 0.99 |
| %CV | 2.73 | 4.40 | 8.64 | 12.20 | 22.57 | 6.43 | 8.78 | 3.77 | 3.05 | 6.60 | 12.45 | 5.50 | 3.96 |
Data represent the mean of the concentration (nmol/106 cells) for the 13 lipid classes
ND not determined
aLinearity ranged from 5 until 50 × 106 cells