Table 3.
Touch-evoked response of zebrafish cacna1aa knockdown larvae
| Treatment | Dose | Normal (%) | Decreased (%) | Absent (%) |
|---|---|---|---|---|
| (n/N) | (n/N) | (n/N) | ||
| Wild type | Uninjected | 97.22 | 2.78 | 0 |
| (35/36) | (1/36) | (0/36) | ||
| Ctrl-MO | 5 ng | 98.07 | 1.92 | 0 |
| (51/52) | (1/52) | (0/52) | ||
| cacna1aa MO1 | 7.5 ng | 73.37* | 18.51* | 11.11* |
| (38/54) | (10/54) | (6/54) | ||
| 9 ng | 0* | 16.66* | 83.33* | |
| (0/24) | (4/24) | (20/24) | ||
| cacna1aa MO2 | 9 ng | 52* | 13.79* | 36.20* |
| (29/58) | (8/58) | (21/58) | ||
| 12 ng | 0* | 5.71 | 94.28* | |
| (0/35) | (2/35) | (33/35) | ||
| cacna1aa MO1 + MO2 | 2.5 ng + 2.5 ng | 96.82 | 3.17 | 0 |
| (61/63) | (2/63) | (0/63) | ||
| 4.5 ng + 4.5 ng | 50* | 20.83* | 29.16* | |
| (12/24) | (5/24) | (7/24) |
n, number of animals with a defined (i.e., absent, decreased, or normal) response; N, total number of animals
Statistical analysis was performed using two-sided Fisher’s exact test
*P < 0.05 vs respective Ctrl-MO group