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. 2020 Apr 2;11(4):215. doi: 10.1038/s41419-020-2409-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a QRT-PCR analysis of UFC1 knockdown efficiency in A549 cells. b Cell counting assays for sh-Ctrl and sh-UFC1 transfected A549 cells. c Colony formation assays for sh-Ctrl and sh-UFC1 transfected A549 cells. d The percentage of apoptotic cells in sh-Ctrl and sh-UFC1 groups was detected by flow cytometry after Annexin V/PI staining. e Flow cytometric analysis of cell cycle distribution in sh-Ctrl and sh-UFC1 transfected A549 cells. f, g QRT-PCR (f) and western blot (g) analysis of cyclin D1, Bcl-2 and Bax expression in sh-Ctrl and sh-UFC1 transfected A549 cells. h Transwell migration and matrigel invasion assays for sh-Ctrl and sh-UFC1 transfected A549 cells. i QRT-PCR analysis of E-cadherin, N-cadherin, vimentin, slug, snail, and twist expression in sh-Ctrl and sh-UFC1 transfected A549 cells. j Western blot analysis of E-cadherin, N-cadherin, vimentin, twist, slug, snail, β-catenin, and c-Myc expression in sh-Ctrl and sh-UFC1 transfected A549 cells. The experiments were repeated for three times. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.