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. 2020 Apr 2;11(4):215. doi: 10.1038/s41419-020-2409-0

Fig. 3. UFC1 overexpression promotes NSCLC cell proliferation, migration and invasion.

Fig. 3

a QRT-PCR analysis of UFC1 overexpression efficiency in H1299 cells. b Cell counting assays for control and UFC1 overexpressing A549 cells. c Colony formation assays for control and UFC1 overexpressing A549 cells. d, e Flow cytometric analysis of cell apoptosis (d) and cell cycle distribution (e) in control and UFC1 overexpressing A549 cells. f, g QRT-PCR (f) and western blot (g) analysis of cyclin D1, Bcl-2 and Bax expression in control and UFC1 overexpressing A549 cells. h Transwell migration and matrigel invasion assays for control and UFC1 overexpressing A549 cells. i QRT-PCR analysis of E-cadherin, N-cadherin, vimentin, snail, slug, and twist expression in control and UFC1 overexpressing A549 cells. j Western blot analysis of E-cadherin, N-cadherin, vimentin, twist, slug, snail, β-catenin, and c-Myc expression in control and UFC1 overexpressing A549 cells.The experiments were repeated for three times. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.