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. 2020 Apr 2;11(4):215. doi: 10.1038/s41419-020-2409-0

Fig. 4. UFC1 interacts with EZH2 to suppress PTEN expression and activate Akt pathway.

Fig. 4

a QRT-PCR analysis of UFC1 expression levels in different subcellular fractions of A549 cells. b RIP assays with antibodies against EZH2 and IgG control and A549 cell extracts. RNA levels in the immunoprecipitates were detected by qRT-PCR. Expression levels of UFC1 are presented as fold enrichment to input. c QRT-PCR analysis of PTEN expression in NSCLC cells with UFC1 knockdown or overexpression. d Western blot analysis of PTEN and p-Akt expression in NSCLC cells with UFC1 knockdown or overexpression. e ChIP-PCR analysis of EZH2 occupancy and H3K27me3 binding in the PTEN promoter in A549 cells transfected with sh-UFC1 and sh-Ctrl. f QRT-PCR and western blot analysis of PTEN expression in A549 cells with EZH2 knockdown. g Correlation analysis of UFC1 and PTEN expression levels in tumor tissues of NSCLC patients. The experiments were repeated for three times. *P < 0.05, **P < 0.01, ***P < 0.001.