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. 2020 Apr 2;10:5804. doi: 10.1038/s41598-020-62730-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Shear stress-mediated changes in expression of SEAP and GFP by SC cells. SC cells were transduced with either eNOS-SEAP or eNOS-GFP adenovirus and subjected to low or high shear stress for 24 hours. (A) SEAP concentration in conditioned media was assessed by a chemiluminescent SEAP assay. Cellular GFP expression was measured using (B) flow cytometry and (C) confocal microscopy. Low shear = 0.1–1 dynes/cm² and high shear = 10–12.5 dynes/cm². Scale bar–50 µm. *P < 0.05., **P < 0.01.