Fig. 3.

DCZ0858 induces cell apoptosis via caspase activation. a NU-DUL-1 and OCI-LY8 cells were treated with DCZ0858 for 48 h, double stained with annexin V-FITC/PI, and analyzed by flow cytometry. b NU-DUL-1 and OCI-LY8 cells were treated with Z-VAD-FMK and DCZ0858 for 48 h, and the cells were analyzed by flow cytometry. c Western blotting was used to analyze the expression levels of the caspase family proteins, namely, caspase-3, caspase-8, caspase-9, and the B-cell lymphoma family proteins (Bcl-2, Bax, and Bcl-xL). All values represent the mean ± SD of three sets of experimental data; *P < 0.05, **P < 0.01, and ***P < 0.001