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. 2020 Apr 2;11:1642. doi: 10.1038/s41467-020-15483-7

Fig. 2. Reduced body weight and improved glucose tolerance in HFD-fed ASK1Δadipo mice.

Fig. 2

a Protein levels of ASK1 in lysate of isolated epididymal adipocytes harvested from ASK1F/F and ASK1Δadipo mice (n = 3 mice per group). b, c Body weight and body weight gain during 12 weeks of chow (ASK1F/F, n = 20 mice; ASK1∆adipo, n = 13 mice) or HFD feeding (ASK1F/F, n = 52 mice; ASK1∆adipo, n = 58 mice). **p = 0.02, ***p < 0.001. d, e Intraperitoneal glucose-tolerance test (chow-fed: ASK1F/F, n = 10 mice; ASK1∆adipo, n = 8 mice; HFD-fed: ASK1F/F, n = 13 mice; ASK1∆adipo, n = 14 mice) at 18 weeks of age. **p = 0.03, ***p < 0.001. f Glucose infusion rate (GIR) during hyperinsulinemic-euglycemic clamps in HFD-fed ASK1F/F (n = 6) and ASK1∆adipo (n = 8) mice. *p = 0.011. g Glucose uptake into respective tissues during hyperinsulinemic-euglycemic clamps in HFD-fed ASK1F/F (n = 5 (ing WAT, epiWAT and skeletal muscle) or n = 6 (BAT)) and ASK1∆adipo (n = 4 (BAT), n = 6 (ingWAT) or n = 7 (epiWAT and skeletal muscle)) mice. *p = 0.034, #p = 0.062. h Endogenous glucose production (EGP) during hyperinsulinemic-euglycemic clamps in HFD-fed ASK1F/F (n = 6) and ASK1∆adipo (n = 8) mice. *p = 0.040. i Liver triglyceride levels determined in HFD-fed ASK1F/F (n = 6) and ASK1∆adipo (n = 6) mice. *p = 0.012. j Mesenteric adipose tissue mRNA expression of respective targets determined in HFD-fed ASK1F/F (n = 5 (Il-10, F4/80) or n = 8 (TNFα, Il-6, Mcp1, Il-1β) and ASK1∆adipo (n = 7 (Il-10, F4/80) or n = 9 (TNFα, Il-6, Mcp1, Il-1β)) mice. Values are expressed as mean ±  SEM. Statistical tests used: two-sided t-tests for (f, g) (without adjustments for multiple comparisons), h, i ANOVA for (b–e). AUC: area under the curve. Source data are provided as a Source Data file.