Fig. 4. Increased adipose tissue browning in HFD-fed ASK1^Δadipo mice.

a, b Fat pad mass as well as total body fat mass (chow-fed: ASK1^F/F, n = 13; ASK1^∆adipo, n = 10; HFD-fed: ASK1^F/F, n = 10; ASK1^∆adipo, n = 16) in mice at 18 weeks of age. **p = 0.005; ***p < 0.001. c Representative image of inguinal adipose tissue of a HFD-fed ASK1^F/F and ASK1^∆adipo mouse (scale bar: 10 mm). d Representative immunohistochemistry for UCP1 in inguinal adipose tissue of a HFD-fed ASK1^F/F (n = 3 mice) and ASK1^∆adipo mouse (n = 3 mice) (scale bar: 50 µm). e mRNA expression of respective genes in inguinal adipose tissue of HFD-fed ASK1^F/F (n = 8) and ASK1^Δadipo (n = 9) mice. *p = 0.027 (Ucp1), *p = 0.028 (Cidea). f, g Representative western blot and quantification of UCP1 and PGC1α protein levels in inguinal adipose tissue harvested from HFD-fed ASK1^F/F (n = 8) and ASK1^Δadipo (n = 10) mice. *p = 0.031 (Ucp1), *p = 0.015 (Cidea). h, i Representative western blot and quantified protein levels of UCP1 in total lysates of inguinal white adipose tissue harvested from cold-exposed mice chronically treated with or without LPS (saline ASK1^F/F, n = 6 mice; saline ASK1^∆adipo, n = 5 mice; LPS ASK1^F/F, n = 7 mice; LPS ASK1^∆adipo, n = 6 mice). **p = 0.003. Values are expressed as mean ±  SEM. Statistical tests used: two-sided t-tests for, e (Cidea), i two-sided Mann–Whitney for e (UCP1), g ANOVA for (a and b). Source data are provided as a Source Data file.