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. 2020 Apr 2;11:1642. doi: 10.1038/s41467-020-15483-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Protein levels of ASK1 in lysate of isolated adipocytes harvested from CAG-ASK1^F/F and CAG-ASK1^+adipo mice (n = 2 mice per group). b mRNA expression of respective genes in inguinal adipose tissue of cold-exposed CAG-ASK1^F/F (n = 5) and CAG-ASK1^+adipo (n = 6) mice. ^#p = 0.094, *p = 0.042, *p = 0.017. c, d Representative western blot and quantification of UCP1 protein levels in inguinal adipose tissue harvested from cold-exposed CAG-ASK1^F/F (n = 7) and CAG-ASK1^+adipo (n = 8) mice. *p = 0.037. Values are expressed as mean ± SEM. Statistical tests used: two-sided t-tests for (b) (Ucp1 and Pgc1α), d two-sided Mann–Whitney for (b) (Cidea). Source data are provided as a Source Data file.