Table 1.
Details of key reagents and resources used.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Ki67 | Abcam | Ab15580 |
| Mouse anti-ki67 | CellSignalling | 9449 |
| Rabbit anti-CC3 | CellSignalling | 9664 |
| Rabbit anti-Sox2 | Abcam | Ab5603 |
| Mouse anti- Nestin | AMD Millipore | Mab5326 |
| Mouse anti-H2a.X | EMD Millipore | 05-636-I |
| Rabbit anti-DCX | Abcam | Ab11267 |
| Mouse anti-Map2 | Abcam | Ab11267 |
| Rabbit anti-NRF2 | Abcam | Ab31163 |
| 555 Donkey Anti-rabbit IgG | Life Technologies | A-31572 |
| 488 Donkey Anti-mouse IgG | Life Technologies | A- 21202 |
| Chemicals, peptides, and recombinant proteins | ||
| Dulbecco’s modified Eagle’s medium nutrient mixture f-12 ham | Sigma | D6421 |
| Human albumin solution | Zenalb | 20 |
| Apo-transferrin | Sigma | T1147 |
| Putrescine DIHCL | Sigma | P5780 |
| Human recombinant insulin | Sigma | I9278-5ml |
| Progesterone | Sigma | P8783 |
| l-glutamine | Sigma | G7513 |
| Sodium selenite | Sigma | S9133-1MG |
| Epidermal growth factor (EGF) | Peprotech | AF 100-15-500 |
| Basic fibroblast growth factor (bFGF) | Peprotech | EC 100-18B |
| 4-hydroxytamoxifen (4-OHT) | Sigma | H7904 |
| Accutase | Sigma | A1110501 |
| Laminin | Sigma | L2020 |
| Penicillin-streptomycin | Life Technologies | P/S |
| Tert-butyl hydroperoxide (tBHP) | Fisher Scientific | 10703571 |
| Hydroxyurea (HU) | Sigma | H8627 |
| TRIreagent | Sigma | T9424 |
| Random hexamers | Life Technologies | N8080127 |
| dNTP mix | Thermo Scientific | RO191 |
| First Strand buffer | Invitrogen | Invitrogen |
| Dithithretiol | Life Technologies | 18080-044 |
| RNaseOUT | Life Technologies | 10777 |
| SuperScript III Reverse Transcriptase | Invitrogen | 18080093 |
| EvaGreen | Solis BioDyne | 08-24-00008 |
| 100 base-pair DNA ladder | Solis BioDyne | 07-11-00050 |
| 1× DNA Loading Dye | Thermo Fisher Scientific | R0611 |
| DAPI | Sigma | D9542-5mg |
| Critical commercial assays | ||
| TURBO DNA-free™ Kit | Life Technologies | AM1907 |
| Deposited data | ||
| Twins UK cohort data | https://twinsuk.ac.uk/resources-for-researchers/access-our-data/ | NA |
| Experimental models: cell lines | ||
| HPC0A07/03A | ReNeuron Ltd | HPC0A07/03A |
| Oligonucleotides | ||
|
mTOR primers Forward TCTTCCATCAGACCCAGTGA Reverse GCTGCCAGCGATCTGAATAA |
This paper | NA |
|
GRB10 primers Forward CACCTGCCTGGCTTCTATTA Reverse TGACTGAGGAGCAGAGAAATG |
This paper | NA |
|
4E-Bp1 primers Forward CGGAAATTCCTGATGGAGTG Reverse CCGCTTATCTTCTGGGCTATT |
This paper | NA |
|
s6K primers Forward CATGAGGCGACGAAGGAG Reverse GGTCCAGGTCTATGTCAAACA |
This paper | NA |
|
eIF4e primers Forward GAAAAACAAACGGGGAGGAC Reverse TCTCCAATAAGGCACAGAAGTG |
This paper | NA |
|
IGF2r primers Forward GAAACAGAGTGGCTGATGGA Reverse CTGAGGGCTTTCACTGACTT |
This paper | NA |
|
IRS2 primers Forward CCACCATCGTGAAAGAGTGAA Reverse CAGTGCTGAGCGTCTTCTT |
This paper | NA |
|
PTEN primers Forward GGTAGCCAGTCAGACAAATTCA Reverse CAACCAGAGTACTACCACCAAAG |
This paper | NA |
|
ETV6 primers Forward AGGCACCATAATCCCTCCCT Reverse GGGGTCTGCAGCTGTTTAGT |
This paper | NA |
|
FoxO3a primers Forward GGAGAGCTGAGACCAGGGTA Reverse AGATTCTCGGCTGACCCTCT |
This paper | NA |
|
Sirt1 primers Forward AGAACCCATGGAGGATGAAAG Reverse TCATCTCCATCAGTCCCAAATC |
This paper | NA |
|
MASH1 primers Forward GCAGCACACGCGTTATAGTA Reverse ACTCGTTTCTAGAGGGCTAAGA |
This paper | NA |
|
UCP2 primers Forward CCTCTACAATGGGCTGGTTG Reverse TCAGAGCCCTTGGTGTAGAA |
This paper | NA |
|
Nrip1 primers Forward GGAGACAGACGAACACTGATATT Reverse GGTCTGTAGCAGTAAGCAGATAG |
This paper | NA |
|
NAMPT primers Forward CCAGGAAGCCAAAGATGTCTAC Reverse GAAGATGCCCATCATACTTCTCA |
This paper | NA |
|
ABTB1 primers Forward ACCATGAACCCGTCCTGA Reverse AAGCAGGCATAGTACCTCCA |
This paper | NA |
|
VIM primers Forward CTTTGCCGTTGAAGCTGCTA Reverse GAAGGTGACGAGCCATTTCC |
This paper | NA |
|
RPLP2 primers Forward CAGAGGAGAAGAAAGATGAGAAGAA Reverse CTTTATTTGCAGGGGAGCAG |
This paper | NA |
|
ACTG1L primers Forward GGCTGAGTGTTCTGGGATTT Reverse GGCCAAAGACATCAGCTAAGA |
This paper | NA |
| Software and algorithms | ||
| QuantStudio 5 Real-Time PCR | ThermoFisher | NA |
| HCS studio software | ThermoScientific | NA |
| Columbus TM Image Data Storage and Analysis System | Perkin Elmer | NA |
| Prism 6 software (GraphPad Software) | GraphPad | NA |
| R Studio | RStudio Desktop | NA |
| CANTAB PAL | Cambridge Cognition | NA |