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. 2020 Apr 2;10:5798. doi: 10.1038/s41598-020-62848-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of the optimized resazurin reduction assay using assay quality control metrics (coefficient of variation (CV), signal window (SW), and Z-factor (Z)). (a) CV, (b) SW, and (c) Z were determined using cell viability data for MCF7, HCC38, MCF-10A, and MDA-MB-436 breast cancer cells treated with bortezomib for 24 hours. Three independent experiments were performed in triplicate. The red dotted lines depict cutoffs set at CV < 20%, SW > 2, and Z > 0.4. The scatterplots were generated using ggplot2 (version 3.2.1) in R⁵¹.