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. 2020 Apr 2;11:1641. doi: 10.1038/s41467-020-15490-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a SepF depletion. Western blots of whole-cell extracts from the P_ino-sepF strain, in the absence (not depleted) or presence (SepF depleted) of 1% myo-inositol during 2 h. SepF and FtsZ levels were revealed using anti-SepF (α-SepF) and anti-FtsZ (α-FtsZ) antibodies. Molecular weight (MW) markers are indicated. b Growth curves of WT cells in 1% myo-inositol (green triangles), P_ino-sepF in the absence (blue circles) or presence (red squares) of 1% myo-inositol. Error bars represent the mean ± SD. c Representative images in phase contrast (upper row) and HADA fluorescent signal (lower row) of P_ino-sepF and WT strains in 1% myo-inositol at time points 0, 3, 6, and 9 h after myo-inositol addition. Heatmaps representing the localization pattern of HADA at 0, 3, and 6 h are shown in Supplementary Fig. 2a; d Violin plot showing the distribution of cell length at time points 0, 3, 6 h after myo-inositol addition for P_ino-sepF (top) and WT (bottom) from c. The number of cells used in the analyses (n) is indicated below each violin representation, triplicate analyses and statistics are shown in Supplementary Fig. 2b and Supplementary Tables 5 and 6. The box indicates the 25th to the 75th percentile and the whiskers indicate the 95% confidence interval. The mean and the median are indicated with a dot and a line in the box, respectively. e Representative images in phase contrast (upper row), HADA fluorescent signal (middle row) and mNeon-FtsZ fluorescent signal (bottom row) at time points 0, 3, 6, and 9 h after myo-inositol addition for cells grown in minimal medium CGXII supplemented with 4% sucrose. f Heatmaps representing the localization pattern of HADA and mNeon-FtsZ at 0, 3, and 6 h. n numbers represent the number of cells used in the analyses. Triplicate analyses for the distribution of cell length at time points 0, 3, 6 h, as well as heatmaps for fluorescence distribution are shown in Supplementary Fig. 5. Scale Bars are 5 μm. Source data are provided as a Source Data file. The data shown are representative of experiments made independently in triplicate.