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. 2020 Apr 1;13(4):100760. doi: 10.1016/j.tranon.2020.100760

Figure 5.

Figure 5

Immunofluorescence of biomarkers in patient-derived CRC spheroids. Representative confocal images (maximum intensity projections of z-stacks) of cancer spheroids stained at day 4 post spheroid embedding in collagen for the (A) CRC biomarkers CK20 and CDX2; (B) cancer stem cell biomarkers ESA and CD133 at day 4; (C) active form of β-catenin, i.e., a molecular marker known to be active in CRC. (D) Representative confocal images of the proliferation marker Ki-67 at day 0 and day 7 (scale bar 100 μm) (− = control; M = MET; A = ASA; C = ASA/MET combination).