Figure 2.

Effect of rifampicin and oxysterols on the cholesterol efflux (upper panels A–C) and the total cholesterol influx (lower panels D–F) in human primary monocyte–macrophage generated foam cells in vitro (normalized by protein content). Experiments without acceptors (panels A, D) and with acceptors (ApoAI, panels B, E; HDL₂, panels C, F) are shown. The experiments were performed in four separate wells per experimental condition (n = 4). The results are presented as mean + SD. 22RHC is a positive control, and DMSO and EtOH are vehicle controls for rifampicin and oxysterols, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001 versus vehicle control; ANOVA with Dunnett’s post hoc test.