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. 2020 Mar 27;11:446. doi: 10.3389/fimmu.2020.00446

Figure 2.

Figure 2

Kinetic analysis of ST2 expression in splenic B cells throughout pregnancy. (A) Representative pseudocolor and contour plots showing gating strategy used to quantify percentages of ST2-expressing B cells in the spleen of pregnant and non-pregnant females. Lymphocytes were gated by FSC vs. SCC, doublets were eliminated, and B220 was used to define total B cell population in the spleen. Fluorescence minus one (FMO) was used as control. (B) Box and whisker plots showing percentages of ST2-expressing total B cells in the spleen of pregnant mice on day 12 (12 dpp; n = 9), 14 (14 dpp; n = 11), and 16 (16 dpp; n = 7) of pregnancy as well as in non-pregnant control females (NP, n = 11). Data are expressed as Box and Whisker plots showing median. *p < 0.05; **p < 0.01; ***p < 0. 001; and ****p < 0.0001 as analyzed by Kruskal–Wallis test followed by Dunn's multiple comparisons test.