Table 1.

Purification of pro-Monalysin.

Fraction		Total protein (mg)	Total activitya (units)	Specific activityb (units/mg protein)	Purification (fold)	Yield (%)
I	Total lysate	3.5	3.1 × 103	8.7 × 102	1.0	100
II	25–50% (NH4)2SO4 pptc	2.6	1.7 × 103	6.8 × 102	0.78	57
III	Anion exchange HPLC	2.3 × 10−1	1.6 × 103	7.0 × 103	8.0	52
IV	0–50% (NH4)2SO4 ppt	6.0 × 10−2	4.7 × 102	8.4 × 103	9.7	16
V	Gel filtration HPLC	2.0 × 10−2	1.8 × 102	1.2 × 104	14	6

^aTotal activity was calculated as 1 unit corresponding to activity that yields 70% cell viability.

^bSpecific activity indicates total activity divided by total protein.

^cppt: precipitate.