Figure 6.

EVs-miR-181b-5p Induces Angiogenesis to Foster ESCC Metastasis In Vivo

(A) The flow diagram for creating the metastatic tumor model. (B) Representative images of liver and lung metastasis of indicated mice treated with EVs derived from Eca-109/miR-181b-5p cells, Eca-109/miR-NC cells, or Eca-109/inh-181b-5p cells were determined by GFP-based fluorescence imaging (n = 5). (C) The number of liver metastatic sites (indicated by arrows) was determined by H&E staining and counted under microscope. Scale bars, 5 mm (left panels); 100 μm (right panels). (D) The number of lung metastatic sites (indicated by arrows) was determined by H&E staining and counted under microscope. Scale bars, 5 mm (left panels); 100 μm (right panels). (E) The livers were performed by immunohistochemistry staining for CD31, and microvessel density was analyzed. Scale bars, 100 μm. (F) The lungs were performed by immunohistochemistry staining for CD31, and microvessel density was analyzed. Scale bars, 100 μm. Each experiment was performed three times independently, and data are presented as mean ± SD. Student’s t test was used to analyze the data (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001).