Table 1.

Description of the NGS pools. All the PCR products (n = 685) were grouped in 12 NGS pools according to the type of skin sample and PCR protocol applied as indicated in the table. For pools 1–3, 2–4, 5–7, 6 both HS and AK specimens of the same individuals gave a PCR product with indicated primers. In contrast the remaining pools include PCR products of unpaired AK (9−10) or HS (11–12), since the matched skin samples were negative with indicated PCR protocols.

NGS pool	PCR protocol	Specimen (AK or HS)∗	Total number
1	FAP59/64	AK	71
3	FAP59/64	HS	71
2	FAP59/64	AK	70
4	FAP59/64	HS	70
5	FAPM1	AK	53
7	FAPM1	HS	53
6	FAPM1	AK	53
8	FAPM1	HS	53
9	FAP59/64	AK	34
10	FAPM1	AK	16
11	FAP59/64	HS	41
12	FAPM1	HS	100

^∗AK, actinic keratosis; HS, healthy skin.