Fig. 3. Effects of Nrf2 knockdown on phosphorylation of ERK and AKT. (A) Western blot analysis. After Nrf2-specific siRNA transfection in SW480, phosphorylation of ERK and AKT was downregulated. In addition, Bcl-2 expression was decreased. β-actin used as a loading control. (B) Densitometric analysis showed relative density of Nrf2, p-ERK, p-AKT, and Bcl-2. si-C, Stealth RNAi control; si-Nrf2, small interfering Nrf2.