Fig. 2.

Most transiently overexpressed eIF3i in CHOK1 cells may not incorporate into the endogenous eIF3 complex. A. Immunofluorescence detection of eIF3i in CHOK1 and HEK293 cells 24 h after transient transfection with eIF3i. Bar = 10 μ. Side panel: western blots of equal amounts of cell lysate protein from CHOK1 and HEK cells probed for eIF3i. Arrowed, a protein weakly detected by the anti-eIF3i antibody on western blot. B. Pulldowns (PDs) with the indicated antibodies were prepared from extracts of CHOK1 and HEK cells transiently transfected as indicated. Western blots of the extracts and PDs were probed with the indicated antibodies.