Fig. 4.

Characterisation of the HEK eIF3i over-expressing cell lines. A. Cell growth of the indicated HEK cell lines as determined using the xCELLigence System. B. The eIF3iV5 HEK cell line showed significantly increased cap-dependent translation of a firefly luciferase reporter compared with the control cell line. Error bars are ± 1 SD and the difference between v and 3iV5 is statistically significant (p < 0.00044). C. Western blot detection of c-Myc in cell lysates of samples taken at sequential days in culture of the indicated cell lines. D. Western blot of cell extracts of the indicated panel of stable eIF3x HEK cell lines probed for eIF3i and α-tubulin as a loading marker.