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. 2020 May;59:98–105. doi: 10.1016/j.ymben.2020.02.001

Table 2.

Primers used for amplification of cDNAs encoding CHO cell CCT subunits.

Gene Target Sequence
CCT1 forward AAGGATCCGTTCCCCGCTGTGGTGGC
CCT1 reverse GCGAGCACAGAATTAATACGACT
CCT2 forward TATGGATCCACTTCCGCCTTCCTTTCCCC
CCT2 reverse AGTTTAAGTAACTGGGTTTTTATTTTAATACAGTCTGAG
CCT3 forward TATGGATCCCTCTTCTCTCCAGAAGCTTCTGCC
CCT3 reverse CAAATGACTTAGATTTAATCATTGGAAGC
CCT4 forward TATGGATCCTCAGCTTCCGCCTCCTCTGC
CCC4 reverse GCAGTTTGGAATCAAATATTTAATATTTTCATTAAATC
CCT5 forward TATGGATCCTAGTCCCGAGCGGTCCGTG
CCT5 reverse TTAACAGTTTCCAAACAGCTGCTTTTATTCCAGC
CCT6 forward TATGGATCCACGTTGTTCTCGGCCTCTCC
CCT6 reverse CTCATCTTAATATGTAATTTTTGTGTCAATGTAG
CCT7 forward TATGGATCCGAGCATTGTGGGCTTGGGTG
CCT7 reverse ATGAGAAAAACCATGACCAAATAATCTTAAG
CCT8 forward TCTTCCTCCGAGCGCGTGAG
CCT8 reverse CGCGGATCCGAATTAATACGACTCACTATAGG