Table 2. Combination of blocking reagents and antibodies used for immunohistochemistry.
| Detection | Blocking reagent | Primary antibody | Secondary antibody |
|---|---|---|---|
| 5-HT | Blocking One Histo | Rabbit polyclonal antibody against 5-HT | EnVision+ System-HRP Labeled Polymer |
| (Nacalai Tesque, Kyoto, Japan) | (20080, 1:80,000) | Anti-Rabbit | |
| (ImmunoStar, Hudson, NJ, USA) | (Dako, Glostrup, Denmark) | ||
| TPH2 | Blocking One Histo | Rabbit polyclonal antibody against TPH2 | EnVision+ System- HRP Labeled Polymer |
| (Nacalai Tesque, Kyoto, Japan) | (PA1-778, 1:4,000) | Anti-Rabbit | |
| (Thermo Fisher, Waltham, MA, USA) | (Dako, Glostrup, Denmark) | ||
| TH | Blocking reagent A and B | Mouse monoclonal antibody against TH | Histofine MAX-PO (M) |
| (Nichirei Bioscience, Tokyo, Japan) | (MAB318; 1:500) | (Histofine Simple Stain system) | |
| (Merck Millipore, Darmstadt, Germany) | (Nichirei Bioscience, Tokyo, Japan) | ||
5-HT: serotonin, TPH2: tryptphan hydroxylase 2, TH: tyrosine hydroxylase.