Table 1. Primary antibodies and result of immunohistochemistry.
Antibody | Clone | Animal | Dilution | Source | Expression by the neoplastic cells | |
---|---|---|---|---|---|---|
Case 1 | Case 2 | |||||
CK | AE1/AE3 | Mouse | × 40 | Abcam, Tokyo, Japan | + | + |
Vimentin | VM452 | Mouse | × 400 | Novus Biologicals, Littleton, CO, USA | + | + |
SMA | Polyclonal | Rabbit | × 200 | Abcam | - | - |
S100 | Polyclonal | Rabbit | × 100 | Thermo Scientific, Fremont, CA, USA | + | + |
NSE | Polyclonal | Rabbit | × 200 | Spring Bioscience, Pleasanton, CA, USA | - | - |
Calretinin | Polyclonal | Rabbit | × 40 | Lsbio, Seattle, WA, USA | - (nuclear) | - (nuclear) |
WT1 | Polyclonal | Rabbit | × 100 | Santa Cruz Bio, Santa Cruz, CA, USA | + | + |
PR | hPRa 2 | Mouse | × 50 | Thermo Scientific | - | - |
ER | Polyclonal | Rabbit | × 100 | Spring Bioscience | - | - |
ALV | Antiserum | Rabbit | × 40,000 | Tsukamoto et al. [27] | - | - |
CK, pan-cytokeratin; SMA, smooth muscle actin; NSE, neuron specific enolase; WT1, Wilms tumor 1; PR, progesterone receptor; ER, estrogen receptor; ALV, avian leukosis virus.