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. 2020 Mar 12;106(4):549–558. doi: 10.1016/j.ajhg.2020.02.011

Figure 3.

Figure 3

Behavioral Response Following PTZ Exposure in sema6ba and sema6bb F0 Crispants

(A) Representative trajectory plots are shown, with the tracking from one 7 dpf larva per well, during exposure to PTZ for 3 min (PTZ concentrations of 0, 2.5, 5, and 15 mM).

(B) Quantification of the swimming distance of 7 dpf larvae during 15 min after treatment with different concentrations of PTZ. Data are represented as the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001 by Student’s t test.

(C) Sample locomotion tracking plots of individual 7 dpf larva. Plots are shown for typical seizure behaviors. A PTZ-induced seizure is characterized in three stages: stage I (increased swim activity), stage II (increased swim activity with circular motion), and stage III (convulsions).

(D) Percentages of 7 dpf larvae in different behavioral categories (stage I, stage II, stage III) with exposure to 2.5, 5, and 15 mM PTZ and observation for 15 min.