Figure 5.

Co-localization and Dual-Luciferase Assay Results

Significance level for testing differential expression for proband and sibling allele in a dual-luciferase assay, versus sum of co-localization local fdr of 10 brain tissues for 51 de novo mutations in ASD probands; mutations with sum of co-localization local fdr below 7 are represented as squares, and labeled with their nearest gene; all other mutations are shown as circles. Significance levels for testing differential expression were computed on the basis of a t test and Fisher’s combined probability test (two sided; gray for p < 0.05, orange for p < 0.01, blue for p < 0.001, red for p < 0.0001).