Figure 1.

Structure-Based Design of Stabilizing Mutations

(A) Schematic structure of filovirus GP showing the GP1 head domain (red, yellow) that includes the mucin-like domain (dashed red/white) and the glycan cap (yellow), GP2 that includes RR1 (with the hinge region in cyan), the base helix (BH, between RR1 and RR2) in green, refolding region 2 (RR2, in purple), and the transmembrane domain (TM, in gray).

(B) Cartoon of the 5JQ3 crystal structure used for the structure-based design. Coloring is as in (A). Amino acid residues shown as a ball-and-stick model were selected for mutagenesis.