| Antibodies |
|
| mAb100 |
Corti et al., 2016 |
N/A |
|
| Chemicals, Peptides, and Recombinant Proteins |
|
| CHT type 1 resin |
Bio-Rad |
Cat# 158-4000 |
| HisTrap HP 5 mL |
GEHC |
Cat#17524802 |
| HiLoad Superdex 200 16/600 size-exclusion column |
GEHC |
Cat# 28989335 |
| mAb Select SuRe 5 mL HiTrap |
GEHC |
Cat# 11003495 |
| HiPrep 26/10 Desalting column |
GEHC |
Cat# 17508701 |
| anti-hIgG (AHC) sensors |
FortéBio |
cat#18-1092 |
| Sypro Orange dye |
Thermo Fisher Scientific |
Cat# S6650 |
|
| Critical Commercial Assays |
|
| 50K Amicon Ultra concentrators |
Millipore |
Cat# UFC905024 |
| NativePage Bis-Tris gradient gels 4-16% |
LifeTechnologies |
Cat# BN1002BOX |
|
| Deposited Data |
|
| Stabilized Makona Δ-mucin GP X-ray structure |
This paper |
PDB: 6VKM
|
|
| Experimental Models: Cell Lines |
|
| Human: Expi293F |
Thermo Fischer Scientific |
Cat#A14527 |
|
| Software and Algorithms |
|
| FortéBio Data Analysis 8.1 software |
FortéBio |
|
| CCP4i interface |
Morin et al., 2013, Potterton et al., 2003
|
https://www.ccp4.ac.uk/ccp4i_main.php |
| iMOSFLM |
Powell et al., 2017 |
https://www.mrc-lmb.cam.ac.uk/mosflm/imosflm/ver730/installation.html |
| AIMLESS |
Evans and Murshudov, 2013 |
http://www.ccp4.ac.uk/dist/html/aimless.html |
| PHASER |
McCoy et al., 2007 |
https://www.phenix-online.org/documentation/reference/phaser_mr.html |
| Coot |
Emsley and Cowtan, 2004 |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| PHENIX |
Adams et al., 2002 |
|
| Astra 7.3 software package |
|
https://www.wyatt.com/products/software/astra.html |
| Chromeleon 7.2.8.0 software package |
|
https://www.thermofisher.com/order/catalog/product/CHROMELEON7 |