Skip to main content
. 2020 Mar 31;30(13):4540–4550.e3. doi: 10.1016/j.celrep.2020.03.025
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

mAb100 Corti et al., 2016 N/A

Chemicals, Peptides, and Recombinant Proteins

CHT type 1 resin Bio-Rad Cat# 158-4000
HisTrap HP 5 mL GEHC Cat#17524802
HiLoad Superdex 200 16/600 size-exclusion column GEHC Cat# 28989335
mAb Select SuRe 5 mL HiTrap GEHC Cat# 11003495
HiPrep 26/10 Desalting column GEHC Cat# 17508701
anti-hIgG (AHC) sensors FortéBio cat#18-1092
Sypro Orange dye Thermo Fisher Scientific Cat# S6650

Critical Commercial Assays

50K Amicon Ultra concentrators Millipore Cat# UFC905024
NativePage Bis-Tris gradient gels 4-16% LifeTechnologies Cat# BN1002BOX

Deposited Data

Stabilized Makona Δ-mucin GP X-ray structure This paper PDB: 6VKM

Experimental Models: Cell Lines

Human: Expi293F Thermo Fischer Scientific Cat#A14527

Software and Algorithms

FortéBio Data Analysis 8.1 software FortéBio
CCP4i interface Morin et al., 2013, Potterton et al., 2003 https://www.ccp4.ac.uk/ccp4i_main.php
iMOSFLM Powell et al., 2017 https://www.mrc-lmb.cam.ac.uk/mosflm/imosflm/ver730/installation.html
AIMLESS Evans and Murshudov, 2013 http://www.ccp4.ac.uk/dist/html/aimless.html
PHASER McCoy et al., 2007 https://www.phenix-online.org/documentation/reference/phaser_mr.html
Coot Emsley and Cowtan, 2004 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PHENIX Adams et al., 2002
Astra 7.3 software package https://www.wyatt.com/products/software/astra.html
Chromeleon 7.2.8.0 software package https://www.thermofisher.com/order/catalog/product/CHROMELEON7