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. 2020 Mar 27;11:373. doi: 10.3389/fphar.2020.00373

Figure 6.

Figure 6

The effect of 4-HNE on mitochondrial ROS levels, succinate accumulation, mitochondrial membrane potential and SDH carbonylation. (A) Representative immunoblots and quantification of 4-HNE-protein adducts under hypoxia/reoxygenation (H/R) (n = 3 samples per group). (B) Representative photographs and quantification of mitochondrial ROS levels under the treatment of 4-HNE (40 μM) and 4-HNE (40 μM) +dimethyl malonate (DMM) (n = 3 samples per group). (C, D) The mitochondrial ROS levels after the addition of succinate or dimethyl malonate (DMM) during hypoxia/reoxygenation (H/R) (n = 4 samples per group). (E) The levels of succinate under the treatment of 4-HNE (n = 3 samples per group). (F) The quantification of mitochondrial membrane potential under the treatment of 4-HNE (40 μM) (n = 3 samples per group). (G) Representative immunoblots and quantification of SDH carbonylation under the treatment of 4-HNE (40 μM) (n = 3 samples per group). Group comparisons were performed by ANOVA with Tukey’s post hoc test or Student’s t-test. Data are presented as mean ± SEM. *P < 0.05, ***P < 0.001 versus Con group; #P < 0.05 versus Con+4-HNE group or H/R group; ###P < 0.001 versus H/R group.